Mouse BCHE Gene cDNA Clone / ORF Clone

BCHE cDNA Clone Datasheet

BCHE cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009738.3
• cDNA Size: 1812
• Gene Synonym: MGC107651; C730038G20Rik; Bche
• Source: Mouse

BCHE cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus butyrylcholinesterase DNA.
• Vector: Cloning Vector: pMD18-T Simple Vector.

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

BCHE cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BCHE cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

BCHE cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGACTC AGCATACCAA GGTAACACAG ACCCACTTCC TCCTATGGAT TCTTCTGCTC
61       TGCATGCCTT TTGGGAAGTC ACACACTGAA GAAGACTTCA TAATTACAAC CAAGACCGGA
121      AGGGTCCGAG GGCTGAGCAT GCCAGTTCTT GGTGGCACGG TGACTGCCTT TCTCGGTATC
181      CCCTATGCAC AACCTCCTCT GGGTAGCCTA AGATTCAAAA AGCCGCAACC CTTAAACAAA
241      TGGCCTGACA TCCATAATGC CACTCAATAT GCAAATTCTT GTTATCAGAA CATAGACCAA
301      GCCTTCCCAG GCTTCCAGGG GTCAGAAATG TGGAATCCAA ACACAAACCT CAGTGAAGAC
361      TGCTTGTATC TGAATGTTTG GATTCCAGTA CCGAAGCCTA AAAATGCCAC TGTCATGGTA
421      TGGATCTATG GTGGTGGCTT TCAAACTGGG ACCTCTTCTC TACCTGTTTA CGATGGGAAG
481      TTTCTAGCTC GTGTTGAAAG AGTTATTGTA GTTTCGATGA ACTATAGGGT AGGTGCTCTA
541      GGATTCCTAG CTTTTCCCGG AAATCCCGAT GCTCCAGGAA ACATGGGTTT ATTTGATCAA
601      CAGTTGGCAC TTCAATGGGT CCAAAGAAAT ATAGCTGCTT TTGGAGGGAA TCCTAAAAGT
661      ATAACGATTT TTGGAGAAAG TGCAGGGGCA GCTTCAGTTA GCTTACATTT GCTCTGCCCC
721      CAAAGTTATC CTTTGTTTAC CAGAGCCATT CTTGAAAGTG GCTCCTCTAA TGCCCCCTGG
781      GCAGTAAAGC ATCCTGAGGA AGCCAGAAAC AGAACCTTGA CCTTAGCTAA ATTTACTGGT
841      TGCTCAAAGG AAAATGAGAT GGAGATGATT AAATGCCTTC GAAGTAAAGA TCCTCAGGAA
901      ATTCTTCGCA ATGAAAGGTT CGTTCTCCCC TCTGATTCCA TCTTATCCAT AAATTTTGGT
961      CCAACAGTGG ATGGCGATTT TCTCACCGAT ATGCCCCACA CACTACTCCA ACTAGGAAAA
1021     GTGAAAAAAG CTCAGATCTT AGTGGGAGTT AACAAAGATG AAGGGACAGC TTTCCTAGTG
1081     TACGGTGCTC CGGGTTTCAG CAAAGACAAT GATAGCCTTA TCACAAGGAA GGAATTTCAA
1141     GAAGGTTTAA ATATGTATTT CCCTGGAGTG AGCAGATTGG GCAAGGAAGC AGTTCTTTTC
1201     TACTACGTGG ACTGGTTAGG TGAGCAGTCA CCAGAAGTCT ACCGTGACGC TTTGGATGAT
1261     GTTATTGGAG ATTACAACAT CATCTGCCCT GCACTGGAGT TTACCAAGAA ATTTGCAGAG
1321     CTTGAAAACA ATGCTTTTTT CTACTTTTTC GAACATCGAT CTTCCAAACT ACCTTGGCCG
1381     GAATGGATGG GAGTGATGCA TGGCTATGAA ATTGAATTTG TGTTTGGCTT ACCTCTGGGA
1441     AGAAGAGTTA ATTATACGAG AGCTGAGGAA ATCTTTAGTC GATCCATAAT GAAAACTTGG
1501     GCAAATTTTG CAAAATATGG ACATCCCAAT GGGACCCAGG GCAATAGCAC AATGTGGCCT
1561     GTCTTCACAA GTACTGAACA AAAATACCTA ACATTGAACA CAGAGAAGTC AAAAATATAC
1621     TCTAAACTTC GTGCTCCCCA ATGTCAGTTC TGGAGACTAT TTTTTCCAAA AGTCTTGGAA
1681     ATGACAGGAG ATATTGATGA AACGGAGCAA GAGTGGAAGG CAGGATTTCA TCGCTGGAGC
1741     AATTACATGA TGGACTGGCA AAATCAATTT AACGATTACA CTAGCAAGAA AGAGAGCTGT
1801     ACAGCTCTCT AA

 

Quality Control:cDNA sequencing results is available upon request.