Rat BCAM Gene cDNA Clone / ORF Clone

BCAM cDNA Clone Datasheet

BCAM cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031752.2
• cDNA Size: 1875
• Gene Synonym: Lu, Bcam
• Source: Rat

BCAM cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus basal cell adhesion molecule DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 705 C/T not causing the amino acid variation.

BCAM cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BCAM cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BCAM cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAACCCC CTGACGCCCG CGCAGGGCTA CTGTGGCTCA CCCTCCTGCT GTCGGGCTAC
61       TCAGGTGCCC AGGCTGAGCT GCACGTGTCC GTTCCCCCAC GGGTGGAGGT GATGCGAGGG
121      GAGCAAATTG CCCTGGACTG CACACCACGG GAGCATCCTG AGAATTACGT GCTGGAGTGG
181      TTGCTTGTTG ATGCTTCCGG GGCTCGACAC CGTCTGGCTT CTGTGGAACC ACAGGGCTCA
241      GAGTTCCTGG GCACAATCCA CAACTCTCGG GGCCGCAGAC CCCCATACAA AATAGACTCC
301      CTTGGGCGTT TGGTGATAGC AGAGGCCCAG GTGGGCGATG AACGGGACTA CGTGTGCGTA
361      GTGAAGGCTG GGGCAGCAGG TACCTCAGAG GCCACTTCAA GTGTCCGTGT GTTTGCAACA
421      CCAGAGGCTA CGGAGGTGGC TCCCAACAAA GGGACGCTGT CCGTGATGGA ACAGTTTGCC
481      CAGGAGATCG CCACCTGCAG CAGCAACAAC GGAAACCCGG TGCCTCGGAT CACATGGTAC
541      CAGAACGGGC AGCGTCTGGA TGTACCCATG GAGCTGAACT CAAAGGGCTA TATGACCAGC
601      CGAACTGTCC GGGAAGCCTC AGGACTGTAT TCCCTGACCA GCACCCTCTA CCTGAGGCTC
661      CACAAAGAGG ATCGGGATGC CAGCTTCCAC TGTGCTGCTC ACTATGACTT GCCCTCAGGC
721      CAACATGGCC GCCTAGACAG CCATACCTTC CGCCTCACCC TGCACTATCC CACGGAGCAT
781      GTAGAATTCT GGGTAGGGAG CCCATCTACC ACAGAGGGCT GGGTACGTGA GGGTGATGCT
841      GTCCAGCTGC TCTGCCAGGG TGATGGCAGC CCCAGCCCGG AGTATTCATT TTTTCGAGAA
901      CAGGGAAACC AGGAGGAACA GCTGAATGTC AACCTCAAGG GAAACTTGAC CCTGGAGGGG
961      GTGCATCGGA GCCAGAGCGG GATCTACGGC TGCAGAGTGG AGGATTACGA TGCTGACGAG
1021     GAGGTGCAAC TTGTCAAGAA GCTAAAGCTG CACGTGGCCT ACCTGGATCC GTTGGAGCTG
1081     AGCGTGCCTG AGGAGTTCTC TGTATTTCTG AACAGCAGTG GCACGGTGGT GAACTGCTCT
1141     GCCAGAGGCC TGCCTGCTCC TATTGTGCGC TGGACCAAGG ATTCTGTGAC CGTTGCAGAT
1201     GGTCCCATAC TCTCTCTTGA CTCTGTGACC TTCGACTCAG CTGGCACGTA CACGTGTGAG
1261     GCGTCCACAC CCACAGTCCC CCTCCTGAGC CGCACACAGA GTTTCCAGCT TGTGGTCCAA
1321     GGAGCTCCAG AGTTAAAACC AAATGAAATA AAACCCAAAT CAGGCACTAG CTGGACGGAA
1381     GGCGATGAAG TCATGCTGAC CTGCTCTGCC CGGGGCTTCC CAGAGCCCAA ACTCACCTGG
1441     AGTCAGCGAG GAGATACTAC TCCAGCAGAG CCGCCCTTCG AGGGTAGAGG CTGGATGAGC
1501     AGCTCGCTGA CCTTGAAAGT CACCAGCGCC CTGAGCCGAG AGGGCGTGTC CTGCGAAGCT
1561     TCTAACATCC ACGGCAAGAA CGGTCACGTG TTCCACTTTG GCTCTGTGGC CCCTCAGACT
1621     GCCCAGGCTG GCGTGGCTGT CATGGCCGTG GCAGTCAGCG TCGGTCTCTT GCTACTCGTA
1681     GTCGCTGCTT TCTACTGCAT GAGACGCAAG GGACGCCCCG GCTGCTGCCA GCGAGCAGAG
1741     AAAGGGGCTC CGCCAGCCAG GGAACCGGAG TTGAGCCACT CCGGGTCAGA GCGTCCAGAA
1801     CACACCGGCC TTCTCATGGG TGGGCCCTCC GGAGGAGGCA GAGGTGGCAA CGGGGGCTTT
1861     GGAGATGAGT GTTGA

 

Quality Control:cDNA sequencing results is available upon request.