Mouse BAMBI Protein (His Tag) (Cytokine)

BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis) Protein Datasheet

BAMBI Protein Product Information

• Synonym: 2610003H06Rik
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse BAMBI (Q9D0L6) (Met 1-Ala 152) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

BAMBI Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 27
• Molecular Mass: The secreted recombinant mouse BAMBI comprises 137 amino acids and has a calculated molecular mass of 15.4 kDa. As a result of glycosylation, the apparent molecular mass of rmBAMBI is approximately 20-25 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: BAMBI protein

BAMBI Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

BAMBI Protein Description

BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein that is a pseudoreceptor of type 1 receptors. BAMBI structurally lacks intracellular serine/threonine kinase domain but with an extracellular domain and a short cytoplasmic region that share sequence similarities with type 1 receptors, whose members have functions in signal transduction in various developing and pathological processes. BAMBI competes with the type 1 receptor, a receptor of the transforming growth factor- beta(TGF- beta) , through functioning as negative regulators of TGF-beta by limiting the signaling range of the TGF-beta family during early embryogenesis. The expression of BAMBI can be induced by accumulated beta- catenin and BMP. The expression level of BAMBI was found aberrantly elevated in most colorectal and hepatocellular carcinomas relative to the corresponding non-cancerous tissues. It suggestes that beta-catenin and TGF-beta interfere growth arrest by inducing the expression of BAMBI, and this may contribute to colorectal and hepatocellular tumorigenesis.

References

1. Sekiya T, et al. (2003) The Journal of Biological Chemistry. 279: 6840-6.
2. Shi YG, et al. (2003) Cell. 113(6): 685-700.
3. Wanninger J, et al. (2011) FEBS Lett. 585(9):1338-44