Mouse BAMBI/NMA Gene cDNA Clone / ORF Clone

BAMBI/NMA cDNA Clone Datasheet

BAMBI/NMA cDNA Clone Product Information

• Gene Bank Ref.ID: NM_026505.2
• cDNA Size: 783
• Gene Synonym: 2610003H06Rik; Bambi
• Source: Mouse

BAMBI/NMA cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus BMP and activin membrane-bound inhibitor, homolog (Xenopus laevis) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 734 T/C resulting in the amino acid Leu substitution by Pro.

BAMBI/NMA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BAMBI/NMA cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BAMBI/NMA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATCGCC ACTCCAGCTA CTTCTTCATC TGGCTGCAGC TGGAGCTCTG CGCCATGGCT
61       GTGCTGCTCA CCAAAGGAGA GATCAGATGC TACTGTGATG CTGCTCACTG TGTGGCAACA
121      GGTTACATGT GTAAGTCTGA GCTTAGTGCC TGCTTCTCCA GACTTCTCGA TCCTCAGAAC
181      ACCAATTCTC CACTCACCCA CGGCTGCCTG GACTCACTTG CAAGCACGGC AGACATCTGC
241      CGAGCCAAAC AGGCCCAAAA CCACTCTGGC CCTGCCATGC CCACTTTGGA ATGCTGTCAC
301      GAAGACATGT GCAATTATCG AGGACTGCAT GACGTCCTCT CTCCTTCCAA GAGCGAAGCC
361      TCAGGACAAG GAAACCGGTA TCAGCATGAC AGCAGCAGAA ACCTCATCAC TAAGATGCAG
421      GAGCTGACTT CCTCCAAAGA ACTGTGGTTC CGCGCAGCTG TGATAGCGGT TCCCATTGCT
481      GGCGGACTGA TCTTGGTGCT GCTCATTATG TTGGCCTTGC GAATGCTTCG AAGTGAGAAC
541      AAGAGGCTGC AGGATGAGCG GCAGCAGATG CTGTCCCGTC TGCACTACAG CTTTCACGGA
601      CACCATTCCA AGAAGGGGCA AGTTGCAAAG TTGGACTTGG AGTGCATGGT GCCTGTCAGT
661      GGGCAGGAGA ACTGCTGTCT GACCTGTGAC AAAATGCGGC AAGCAGAGCT CAGTAATGAG
721      AAGATCCTCT CCCCTGTGCA CTGGGGCATG TACAGTGGTC ATGGAAAGCT GGAATTCATA
781      TGA

 

Quality Control:cDNA sequencing results is available upon request.