BAMBI / NMA Protein (His Tag)

BMP and Activin Membrane-Bound Inhibitor Homolog Protein

BAMBI / NMA Protein Product Information

• Synonym: NMA, BAMBI
• Protein Construction: A DNA sequence encoding the human BAMBI extracellular domain (NP_036474.1) ( Met 1- Ala 152 ) was expressed, fused with a C-terminal polyhistidine tag
• Source: Human
• Expression Host: Human Cells

BAMBI / NMA Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 21
• Molecular Mass: The secreted recombinant human BAMBI comprises 143 amino acids with a predicted molecular mass of 16.1 kDa. As a result of different glycosylation, the apparent molecular mass of rh BAMBI is approximately 16-23 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: BAMBI protein

BAMBI / NMA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

BAMBI / NMA Protein Description

BMP and activin membrane-bound inhibitor homolog, also known as BAMBI and NMA, is a single-pass type I  membrane protein which belongs to the BAMBI family. Members of the transforming growth factor-beta (TGF-beta) superfamily, including TGF-beta, bone morphogenetic proteins (BMPs), activins and nodals, are vital for regulating growth and differentiation. These growth factors transduce their signals through pairs of transmembrane type I  and type I I receptor kinases. BAMBI is a transmembrane glycoprotein related to the transforming growth factor-beta (TGF-beta)-family type I  receptors and functions as a negative regulator of TGF-beta signaling during development. BAMBI is co-expressed with the ventralizing morphogen BMP4 during Xenopus embryogenesis and that it requires BMP signalling for its expression. BAMBI is induced by BMP signaling through the evolutionary conserved BMP-responsive elements in its promoter. Wnt/beta-catenin signaling activates transcription of BAMBI and that BAMBI expression is aberrantly elevated in most colorectal carcinomas. BAMBI stably associates with TGF-beta-family receptors and inhibits BMP and activin as well as TGF-beta signalling. BAMBI negatively regulates TGF-beta-family signalling by a regulatory mechanism involving the interaction of signalling receptors with a pseudoreceptor.

References

1. Onichtchouk,D. et al., 1999, Nature. 401 (6752):480-5.
2. Grotewold,L. et al., 2001,Mech Dev. 100 (2):327-30.
3. Sekiya,T. et al., 2004, Biochem Biophys Res Commun. 320 (3):680-4.
4. Gonzales,C.B. et al., 2010,J Dent Res  89 (6):597-602.