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BAMBI / NMA ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10890 |
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Human BAMBI / NMA ELISA Pair Set
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Order or Inquire for BAMBI / NMA ELISA Pair Set |
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High affinity Human BAMBI / NMA ELISA Pair Set |
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Detection limit - 9.375 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human BAMBI / NMA ELISA Pair Set Related Products & Topics
Related Areas:
Cancer>>Growth Factor & Receptor>>TGF-beta Superfamily>>BMP Family>>BAMBI/NMA
Cancer>>Growth Factor & Receptor>>TGF-beta Superfamily>>TGF-beta Family>>BAMBI/NMA
Immunology>>Cytokine & Receptor>>TGF-beta Superfamily>>TGF-beta Superfamily Modulators>>BAMBI/NMA
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
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BAMBI/NMA |
Human |
10890-H08H |
Antibodies:
| Molecule | Application | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| Human BAMBI/NMA |
WB | BAMBI/NMA Antibody, Mouse MAb | 10890-MM04 |
| Human BAMBI/NMA |
ELISA | BAMBI/NMA Antibody, Mouse MAb | 10890-MM06 |
| Human BAMBI/NMA |
WB, ELISA | BAMBI/NMA Antibody, Rabbit PAb | 10890-RP01 |
| Human BAMBI/NMA |
WB, ELISA | BAMBI/NMA Antibody, Rabbit PAb (Antigen Affinity Purified) | 10890-RP02 |
Background
BMP and activin membrane-bound inhibitor homolog, also known as BAMBI and NMA, is a single-pass type I membrane protein which belongs to the BAMBI family. Members of the transforming growth factor-beta (TGF-beta) superfamily, including TGF-beta, bone morphogenetic proteins (BMPs), activins and nodals, are vital for regulating growth and differentiation. These growth factors transduce their signals through pairs of transmembrane type I and type II receptor kinases. BAMBI is a transmembrane glycoprotein related to the transforming growth factor-beta (TGF-beta)-family type I receptors and functions as a negative regulator of TGF-beta signaling during development. BAMBI is co-expressed with the ventralizing morphogen BMP4 during Xenopus embryogenesis and that it requires BMP signalling for its expression. BAMBI is induced by BMP signaling through the evolutionary conserved BMP-responsive elements in its promoter. Wnt/beta-catenin signaling activates transcription of BAMBI and that BAMBI expression is aberrantly elevated in most colorectal carcinomas. BAMBI stably associates with TGF-beta-family receptors and inhibits BMP and activin as well as TGF-beta signalling. BAMBI negatively regulates TGF-beta-family signalling by a regulatory mechanism involving the interaction of signalling receptors with a pseudoreceptor.
