Anti-BAMBI Antibody

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Anti-BAMBI Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-BAMBI Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human BAMBI
Immunogen
Recombinant Human BAMBI protein (Catalog#10890-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human BAMBI (rh BAMBI; Catalog#10890-H08H; NP_036474.1; Met 1-Ala 152). BAMBI specific IgG was purified by human BAMBI affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-BAMBI Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-BAMBI Antibody (Rabbit Polyclonal antibody) Images

Anti-BAMBI rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:26 kDa

Anti-BAMBI Antibody: Alternative Names

Anti-NMA Antibody

BAMBI Background Information

BMP and activin membrane-bound inhibitor (BAMBI) is a transmembrane glycoprotein that is a pseudoreceptor of type 1 receptors. BAMBI structurally lacks intracellular serine/ threonine kinase domain but with an extracellular domain and a short cytoplasmic region that share sequence similarities with type 1 receptors, whose members have functions in signal transduction in various developing and pathological processes. BAMBI competes with the type 1 receptor, a receptor of the transforming growth factor-beta (TGF-beta), through functioning as negative regulators of TGF-beta by limiting the signaling range of the TGF-beta family during early embryogenesis. The expression of BAMBI can be induced by accumulated beta-catenin and BMP. The expression level of BAMBI was found aberrantly elevated in most colorectal and hepatocellular carcinomas relative to the corresponding non-cancerous tissues. It suggestes that beta-catenin and TGF-beta interfere growth arrest by inducing the expression of BAMBI, and this may contribute to colorectal and hepatocellular tumorigenesis.
Full Name
BMP and activin membrane-bound inhibitor
References
  • Sekiya T, et al. (2003) Identification of BMP and Activin Membrane-bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor- Signaling, as a Target of the -Catenin Pathway in Colorectal Tumor Cells. The Journal of Biological Chemistry. 279:6840-6.
  • Shi YG, et al. (2003) Mechanisms of TGF- Signaling from Cell Membrane to the Nucleus. Cell. 113(6): 685-700.
  • Wanninger J, et al. (2011) Adiponectin induces the transforming growth factor decoy receptor BAMBI in human hepatocytes. FEBS Lett. 585(9):1338-44.
  • FOXA1 Reprograms the TGF-β-Stimulated Transcriptional Program from a Metastasis Promoter to a Tumor Suppressor in Nasopharyngeal Carcinoma
    Author
    Li, J;Wang, W;Chen, S;Cai, J;Ban, Y;Peng, Q;Zhou, Y;Zeng, Z;Li, X;Xiong, W;Li, G;Yi, M;Xiang, B;
    Year
    2018
    Journal
    Cancer Letters
    Application
    WB
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