BAMBI / NMA Antibody (Antigen Affinity Purified)

BAMBI / NMA Antibody ( Antigen Affinity Purified )

BAMBI / NMA Antibody Product Information

• Immunogen: Recombinant human BAMBI protein ( Catalog#10890-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human BAMBI ( rh BAMBI ; Catalog#10890-H08H ; NP_036474.1 ; Met 1 - Ala 152 ). BAMBI specific IgG was purified by human BAMBI affinity chromatography

BAMBI / NMA Antibody Usage Guide

• Specificity: Human BAMBI / NMA
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect BAMBI in WB. Using a DAB detection system, the detection limit for human BAMBI is approximately 8 ng/lane under non-reducing conditions and 16 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human BAMBI. The detection limit for human BAMBI is approximately 0.00975ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

BAMBI / NMA Antibody Background

BMP and activin membrane-bound inhibitor homolog, also known as BAMBI and NMA, is a single-pass type I membrane protein which belongs to the BAMBI family. Members of the transforming growth factor-beta (TGF-beta) superfamily, including TGF-beta, bone morphogenetic proteins (BMPs), activins and nodals, are vital for regulating growth and differentiation. These growth factors transduce their signals through pairs of transmembrane type I and type II receptor kinases. BAMBI is a transmembrane glycoprotein related to the transforming growth factor-beta (TGF-beta)-family type I receptors and functions as a negative regulator of TGF-beta signaling during development. BAMBI is co-expressed with the ventralizing morphogen BMP4 during Xenopus embryogenesis and that it requires BMP signalling for its expression. BAMBI is induced by BMP signaling through the evolutionary conserved BMP-responsive elements in its promoter. Wnt/beta-catenin signaling activates transcription of BAMBI and that BAMBI expression is aberrantly elevated in most colorectal carcinomas. BAMBI stably associates with TGF-beta-family receptors and inhibits BMP and activin as well as TGF-beta signalling. BAMBI negatively regulates TGF-beta-family signalling by a regulatory mechanism involving the interaction of signalling receptors with a pseudoreceptor.

References

1. Onichtchouk,D. et al., 1999, Nature. 401 (6752):480-5.
2. Grotewold,L. et al., 2001,Mech Dev. 100 (2):327-30.
3. Sekiya,T. et al., 2004, Biochem Biophys Res Commun. 320 (3):680-4.
4. Gonzales,C.B. et al., 2010,J Dent Res  89 (6):597-602.