BAFF / TNFSF13B Protein (Native) (Cytokine)

Tumor necrosis factor (ligand) superfamily, member 13b Protein Datasheet

BAFF / TNFSF13B Protein Product Information

• Synonym: TNFSF13B ,BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK,TNFSF20, ZTNF4
• Protein Construction: A DNA sequence encoding the soluble form of human BAFF (Q9Y275-1) (Ala 134-Leu 285) was expressed and purified.
• Source: Human
• Expression Host: E.coli

BAFF / TNFSF13B Protein QC Testing

• Purity: > 88 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human BAFF consisting of 153 amino acids and has a calculated molecular mass of 17.2 KDa. It migrates as an approximately 14KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: BAFF / TNFSF13B protein

BAFF / TNFSF13B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

BAFF / TNFSF13B Protein Description

Tumor necrosis factor ligand superfamily member 13B (TNFSF13B), also known as B cell-activating factor, CD257 and BAFF, is single-pass type I I membrane protein. It belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression of BAFF is found in placenta, heart, lung, fetal liver, thymus, and pancreas. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR). These receptors are expressed mainly on mature B lymphocytes, and TACI binds with lowest affinity. TACI binds a protein similar to BAFFcalled A Proliferation Inducing Ligand (APRIL) with high. BCMA displays an intermediate binding phenotype and performs functions with either BAFF or APRIL to varying degrees. BAFF acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells.

References

1. Shu HB. et al., 1999, J Leukoc Biol. 65 (5): 680-3.
2. Schneider P. et al., 1999, J Exp Med. 189 (11): 1747-56.
3. Wu Y. et al., 2000, J Biol Chem. 275 (45): 35478-85.
4. Oren DA. et al., 2002, Nat Struct Biol. 9: 288-92.
5. Daridon C. et al., 2008, Autoimmun Rev. 7 (4): 267-71.