Bad cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10020-M-F
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Bad cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
507 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human BCL2-antagonist of cell death (BAD), transcript variant 1 DNA.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV/hygro-FLAG
Restriction Sites
KpnI + XhoI
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
Bad cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Bad cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human Bad Gene Expression validated Image 2378
The plasmid was transfected into 293E adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope.
Human G-CSF/CSF3 Gene Plasmid Map 7153
Bad cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
BBC2 cDNA ORF Clone, Human;BCL2L8 cDNA ORF Clone, Human
Bad Background Information
Full Name
BCL2-associated agonist of cell death

Bad cDNA ORF Clone, Rat, Related Products

Product Name Catalog#(PDF)
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Bad cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
Bad cDNA ORF Clone in Cloning Vector, Mouse MG51444-G
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Bad Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51444-ACGLN
Bad cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51444-ANG
Bad cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51444-ACR
Bad cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51444-ANR
Bad cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51444-CF
Bad cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51444-NF
Bad cDNA ORF Clone, Mouse, C-His tag MG51444-CH
Bad cDNA ORF Clone, Mouse, N-His tag MG51444-NH
Bad cDNA ORF Clone, Mouse, C-HA tag MG51444-CY
Bad cDNA ORF Clone, Mouse, N-HA tag MG51444-NY
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Bad cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
Bad cDNA ORF Clone in Cloning Vector, Human HG10020-M
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Bad Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10020-ACGLN
Bad cDNA ORF Clone, Human, N-GFPSpark® tag HG10020-ANG
Bad cDNA ORF Clone, Human, C-OFPSpark® tag HG10020-ACR
Bad cDNA ORF Clone, Human, N-OFPSpark® tag HG10020-ANR
Bad cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10020-CF
Bad cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10020-NF
Bad cDNA ORF Clone, Human, C-His tag HG10020-CH
Bad cDNA ORF Clone, Human, N-His tag HG10020-NH
Bad cDNA ORF Clone, Human, C-HA tag HG10020-CY
Bad cDNA ORF Clone, Human, N-HA tag HG10020-NY
Bad cDNA ORF Clone, Human, C-Myc tag HG10020-CM
Bad cDNA ORF Clone, Human, N-Myc tag HG10020-NM
Bad qPCR Primer Pairs, Human HP100117
info info
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