Mouse BACE2 Gene cDNA Clone / ORF Clone

BACE2 cDNA Clone Datasheet

BACE2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_019517.4
• cDNA Size: 1545
• Gene Synonym: ARP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik; Bace2
• Source: Mouse

BACE2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus beta-site APP-cleaving enzyme 2 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 32 C/T and 109 C/G resulting in the amino acid Pro substitution by Leu,Arg substitution by Gly.

BACE2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BACE2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

BACE2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCGCGC TGCTTCGAGC ACTCTTGCTC CTGGTGCTGG CGCAGTGGCT CTTGAGTGCG
61       GTCCCCGCGC TGGCCCCCGC GCCCTTCACG CTGCCCCTCC AAGTGGCCGG GGCCACGAAC
121      CACAGAGCCT CGGCTGTTCC CGGACTCGGG ACCCCCGAGT TGCCCCGGGC CGATGGTCTG
181      GCCCTCGCAC TGGAGCCTGT CAGGGCTACT GCCAACTTCT TGGCTATGGT GGACAACCTT
241      CAGGGGGACT CTGGCCGCGG CTACTACCTA GAGATGCTGA TCGGGACCCC TCCGCAGAAG
301      GTACAGATTC TTGTGGACAC TGGAAGCAGT AACTTCGCTG TGGCAGGTGC CCCACACTCC
361      TACATAGACA CCTACTTTGA CTCAGAGAGC TCCAGCACAT ACCACTCCAA GGGCTTTGAT
421      GTCACTGTGA AGTACACACA GGGAAGCTGG ACTGGCTTTG TTGGTGAGGA CCTTGTCACC
481      ATCCCAAAAG GCTTCAACAG CTCTTTCTTG GTCAATATTG CCACTATTTT CGAGTCTGAG
541      AATTTCTTTT TGCCTGGTAT TAAATGGAAT GGAATCCTTG GACTTGCTTA TGCTGCTTTG
601      GCCAAGCCAT CAAGCTCTCT GGAGACATTT TTTGATTCCC TGGTGGCCCA AGCAAAGATT
661      CCAGACATTT TCTCCATGCA GATGTGCGGG GCTGGATTGC CAGTAGCTGG TTCTGGTACC
721      AACGGAGGTA GTCTTGTCCT GGGTGGGATT GAACCAAGTT TGTATAAAGG AGATATCTGG
781      TATACCCCAA TTAAAGAGGA ATGGTACTAT CAAATAGAAA TCCTGAAGTT GGAAATTGGA
841      GGCCAGAACC TCAACCTGGA CTGCAGAGAG TATAACGCAG ACAAGGCCAT TGTGGACAGT
901      GGCACCACGC TCCTGCGCCT GCCCCAGAAG GTGTTTGATG CAGTGGTGGA AGCTGTGGCA
961      CGAACATCTC TGATTCCAGA GTTTTCTGAT GGCTTCTGGA CAGGGGCCCA GCTGGCATGC
1021     TGGACAAATT CTGAAACGCC ATGGGCATAT TTCCCTAAGA TTTCTATCTA CCTGAGAGAT
1081     GAGAATGCCA GTCGCTCCTT CCGGATCACC ATTCTCCCAC AGCTCTACAT TCAGCCCATG
1141     ATGGGAGCTG GTTTCAATTA TGAATGCTAC CGTTTTGGTA TCTCCTCTTC CACAAATGCG
1201     CTGGTGATTG GTGCGACCGT GATGGAAGGC TTCTACGTGG TCTTTGACAG AGCTCAGAGG
1261     AGGGTGGGCT TTGCAGTGAG TCCCTGTGCA GAGATTGAAG GTACCACAGT GTCTGAAATT
1321     TCTGGGCCCT TTTCCACGGA AGACATAGCC AGCAACTGTG TTCCAGCACA GGCTCTGAAT
1381     GAGCCCATCT TGTGGATTGT GTCCTATGCC CTGATGAGTG TGTGTGGAGC CATTCTCCTG
1441     GTTCTGATCC TCCTCCTGCT GCTCCCGCTG CACTGCCGTC ATGCCCCCCG AGACCCTGAG
1501     GTAGTTAACG ATGAGTCCTC ACTAGTCAGA CATCGCTGGA AATGA

 

Quality Control:cDNA sequencing results is available upon request.