BACE1 Protein & Antibody (ASP2)

Beta-site APP Cleaving Enzyme 1 (Aspartyl Protease 2)

BACE1 Products

BACE1 Protein, Recombinant

Molecule	Species	Description	Cat. No
BACE1/ASP2	Human	BACE1/ASP2/Fc Protein, Recombinant	10064-H02H
BACE1/ASP2	Human	BACE1/ASP2 Protein, Recombinant	10064-H08H
BACE1/ASP2	Human	BACE1/ASP2 Protein, Recombinant	10064-HCCH
BACE1/ASP2	Mouse	BACE1/ASP2 Protein, Recombinant	50002-M08H

  10064-H08H:  The specific activity is > 1.5 pmoles / min / µg. Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog#ES004, R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively.

  10064-HCCH:  The specific activity is > 0.5 pmoles / min / µg. Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog#ES004, R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively.

  50002-M08H:  The specific activity is > 2 pmoles / min / µg. Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dpn)-Arg-Arg-NH2 (Catalog#ES004, R&D Systems). Cleavage of ES004 can be measured using excitation and emission wavelengths of 320 and 405 nm, respectively.

BACE1 Antibody

Molecule	Application	Description	Cat. No
Human BACE1/ASP2	ELISA	Mouse Monoclonal Antibody	10064-MM01
Human BACE1/ASP2	WB, ELISA	Rabbit Monoclonal Antibody	10064-R001
Human BACE1/ASP2	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10064-RP03

BACE1 Related Areas

Enzyme>>Protease & Regulator>>Aspartate Protease & Regulator>>BACE1/ASP2

BACE1 Alternative Names

BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149 [Homo sapiens]

Bace1, C76936 [Mus musculus]

BACE1 Background

Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

BACE1 Related Studies

1. Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74.
2. Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer's disease brain. Biochem Soc Trans. 35(Pt 3): 574-6.
3. Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90.
4. Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.