Anti-BACE1 Antibody

Cat: 10064-R001
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Anti-BACE1 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-BACE1 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human BACE1
Has cross-reactivity in ELISA with Mouse BACE1 / ASP2
No cross-reactivity in ELISA with Human ADAM15
Immunogen
Recombinant Human BACE1 protein (Catalog#10064-HCCH)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human BACE1 extracellular domain (rh BACE1; Catalog#10064-HCCH; Met 1-Thr 457; NP_036236.1) .
Source
Monoclonal Rabbit IgG Clone #1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-BACE1 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BACE1 Antibody Alternative Names
Anti-ASP2 Antibody;Anti-BACE Antibody;Anti-HSPC104 Antibody
BACE1 Background Information

Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.

Full Name
beta-site APP-cleaving enzyme 1
References
  • Christensen MA, et al. (2004) Transcriptional regulation of BACE1, the beta-amyloid precursor protein beta-secretase, by Sp1. Mol Cell Biol. 24(2):865-74.
  • Stockley JH, et al. (2007) The proteins BACE1 and BACE2 and beta-secretase activity in normal and Alzheimer's disease brain. Biochem Soc Trans. 35(Pt 3): 574-6.
  • Savonenko AV, et al. (2008) Alteration of BACE1-dependent NRG1/ErbB4 signaling and schizophrenia-like phenotypes in BACE1-null mice. Proc Natl Acad Sci U S A. 105(14): 5585-90.
  • Hayley M, et al. (2009) Calcium enhances the proteolytic activity of BACE1: An in vitro biophysical and biochemical characterization of the BACE1-calcium interaction. Biochim Biophys Acta. 1788(9): 1933-8.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BACE1 Antibody Rabbit Polyclonal WB,ELISA,IHC-P 50002-T48
    Anti-BACE1 Antibody Rabbit Polyclonal ELISA 50002-RP01
    Anti-BACE1 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 10064-R027
    Anti-BACE1 Antibody (FITC) Rabbit Monoclonal FCM 10064-R027-F
    Anti-BACE1 Antibody (PE) Rabbit Monoclonal FCM 10064-R027-P
    Anti-BACE1 Antibody Rabbit Polyclonal ELISA 10064-RP03
    Anti-BACE1 Antibody Rabbit Polyclonal ELISA 10064-T16
    Anti-BACE1 Antibody Mouse Monoclonal ELISA 10064-MM01
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