Mouse B7X / B7H4 / B7S1 / VTCN1 Protein

V-set domain containing T cell activation inhibitor 1 Protein Datasheet

B7X / B7H4 / B7S1 / VTCN1 Protein Product Information

• Synonym: Vtcn1 ，B7h4, B7s1, B7x, BC032925
• Protein Construction: A DNA sequence encoding the mature form of mouse B7H4 (Q7TSP5) (phe 29-Ser 256) was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

B7X / B7H4 / B7S1 / VTCN1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: phe 29
• Molecular Mass: The secreted recombinant mouse B7H4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 469 amino acids and has a calculated molecular mass of 52.2 kDa. The apparent molecular mass of rmB7H4/Fc monomer is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: B7X / B7H4 / B7S1 / VTCN1 protein

B7X / B7H4 / B7S1 / VTCN1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

B7X / B7H4 / B7S1 / VTCN1 Protein Description

V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass typeⅠ membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.

References

1. Zang X. et al., 2003, Proc Natl Acad Sci. 100 (18): 10388-92.
2. Suh WK. et al., 2006, Mol Cell Biol. 26 (17): 6403-11.
3. Zang X. et al., 2007, Proc Natl Acad Sci. 104 (49): 19458-63.