Mouse CD276 Gene cDNA Clone / ORF Clone

CD276 cDNA Clone Datasheet

CD276 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_133983.4
• cDNA Size: 951
• Gene Synonym: B7H3
• Source: Mouse

CD276 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus CD276 antigen DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD276 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD276 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD276 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTTCGAG GATGGGGTGG CCCCAGTGTG GGTGTGTGTG TGCGCACAGC ACTGGGGGTG
61       CTGTGCCTCT GCCTCACAGG AGCTGTGGAA GTCCAGGTCT CTGAAGACCC CGTGGTGGCC
121      CTGGTGGACA CGGATGCCAC CCTACGCTGC TCCTTTTCCC CAGAGCCTGG CTTCAGTCTG
181      GCACAGCTCA ACCTCATCTG GCAGCTGACA GACACCAAAC AGCTGGTGCA CAGCTTCACG
241      GAGGGCCGGG ACCAAGGCAG TGCCTACTCC AACCGCACAG CGCTCTTCCC TGACCTGTTG
301      GTGCAAGGCA ATGCGTCCTT GAGGCTGCAG CGCGTCCGAG TAACCGACGA GGGCAGCTAC
361      ACCTGCTTTG TGAGCATCCA GGACTTTGAC AGCGCTGCTG TTAGCCTGCA GGTGGCCGCC
421      CCCTACTCGA AGCCCAGCAT GACCCTGGAG CCCAACAAGG ACCTACGTCC AGGGAACATG
481      GTGACCATCA CGTGCTCTAG CTACCAGGGC TATCCGGAGG CCGAGGTGTT CTGGAAGGAT
541      GGACAGGGAG TGCCCTTGAC TGGCAATGTG ACCACATCCC AGATGGCCAA CGAGCGGGGC
601      TTGTTCGATG TTCACAGCGT GCTGAGGGTG GTGCTGGGTG CTAACGGCAC CTACAGCTGC
661      CTGGTACGCA ACCCGGTGTT GCAGCAAGAT GCTCACGGCT CAGTCACCAT CACAGGGCAG
721      CCCCTGACAT TCCCCCCTGA GGCTCTGTGG GTAACCGTGG GGCTCTCTGT CTGTCTTGTG
781      GTACTACTGG TGGCCCTGGC TTTCGTGTGC TGGAGAAAGA TCAAGCAGAG CTGCGAGGAG
841      GAGAATGCAG GTGCCGAGGA CCAGGATGGA GATGGAGAAG GATCCAAGAC AGCTCTACGG
901      CCTCTGAAAC CCTCTGAAAA CAAAGAAGAT GACGGACAAG AAATTGCTTG A

 

Quality Control:cDNA sequencing results is available upon request.