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B7-H3 / CD276 Antibody, Mouse MAb

  • B7-H3 / CD276 Antibody, Mouse MAb, Immunofluorescence
  • B7-H3 / CD276 Antibody, Mouse MAb, Flow cytometric analysis
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Human B7-H3/CD276 Antibody Product Information
Immunogen:Recombinant Human B7-H3 / CD276 protein (Catalog#11188-H08H)
Clone ID:06
Ig Type:Mouse IgG2b
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#11188-H08H; Q5ZPR3-1; Met 1-Thr 461). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human B7-H3/CD276 Antibody IF Application Image
B7-H3 / CD276 Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human B7H3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human B7H3 monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human B7-H3/CD276 Antibody FC Application Image
B7-H3 / CD276 Antibody, Mouse MAb, Flow cytometric analysis
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human B7H3(CD276) expression on PC-3 cells. Cells were stained with purified anti-Human B7H3(CD276), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other B7-H3/CD276 Antibody Products
Reactivity: Human  
Application: ELISA  IP  
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11188-RP02-50
11188-RP02-100
11188-RP02-200
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100 µg 
200 µg 
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Reactivity: Human  
Application: FCM  
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11188-MM06-F-50
11188-MM06-F-100
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Reactivity: Human  
Application: FCM  
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11188-MM06-P-50
11188-MM06-P-100
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Reactivity: Human  
Application: FCM  
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11188-MM06-A-100
11188-MM06-A-50
100 Tests 
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Reactivity: Mouse  
Application: ELISA  WB  IP  
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50973-T52-50
50973-T52-200
50973-T52-100
50 µg 
200 µg 
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Reactivity: Mouse  
Application: ELISA  
    50973-RP01-100
    50973-RP01-400
    50973-RP01-200
    100 µg 
     
    200 µg 
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    Reactivity: Rat  
    Application: ELISA  
      80380-RP01-400
      80380-RP01-200
      80380-RP01-100
       
      200 µg 
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      Reactivity: Rat  
      Application: ELISA  WB  IP  
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      80380-T52-200
      80380-T52-100
      80380-T52-50
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      B7-H3/CD276 Background

      B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

      Immune Checkpoint
      Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
      Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

      Immunotherapy   Cancer Immunotherapy   Targeted Therapy

      Human B7-H3/CD276 References
    • Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
    • Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
    • Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
    • Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
    • Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
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      Catalog: 11188-MM06-50
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      Datasheet & Documentation

      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"