Anti-B7-H3 Antibody

Cat: 11188-MM06
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All B7-H3 Reagents
CRO Services
Product recommended

All B7-H3 Reagents
Anti-B7-H3 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-B7-H3 Antibody
Validated applications
ELISA,ELISA(Det),FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human B7-H3
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Immunogen
Recombinant Human B7-H3 / CD276 protein (Catalog#11188-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#11188-H08H; Q5ZPR3-1; Met 1-Thr 461). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #06
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-B7-H3 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:50-1:1000  
FCM 1:25-1:100  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human B7-H3 / CD276 in ELISA pair set (Catalog: # SEK11188). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11188-MM01).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-B7-H3 Antibody (Mouse Monoclonal antibody) Images
Human B7-H3/CD276 Immunofluorescence(IF) 3004
Immunofluorescence staining of Human B7H3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human B7H3 monoclonal antibody (1:100) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human B7-H3/CD276 Flow Cytometry (FC) 5338
Flow cytometric analysis of Human B7H3(CD276) expression on PC-3 cells. Cells were stained with purified anti-Human B7H3(CD276), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-B7-H3 Antibody Alternative Names
Anti-4Ig-B7-H3 Antibody;Anti-B7-H3 Antibody;Anti-B7H3 Antibody;Anti-B7RP-2 Antibody
B7-H3 Background Information

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD276 molecule
References
  • Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
  • Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
  • Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
  • Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
  • Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-B7-H3 Antibody Rabbit Polyclonal ELISA 80380-RP01
    Anti-B7-H3 Antibody Rabbit Polyclonal WB,ELISA,IP 80380-T52
    Anti-B7-H3 Antibody Rabbit Polyclonal ELISA 50973-RP01
    Anti-B7-H3 Antibody Rabbit Polyclonal WB,ELISA,IP 50973-T52
    Anti-B7-H3 Antibody Mouse Monoclonal ELISA 11188-M008
    Anti-B7-H3 Antibody Mouse Monoclonal ELISA(Cap) 11188-MM01
    Anti-B7-H3 Antibody (FITC) Mouse Monoclonal FCM 11188-MM06-F
    Anti-B7-H3 Antibody Rabbit Polyclonal ELISA,IHC-P 11188-T24
    Anti-B7-H3 Antibody Rabbit Polyclonal ELISA,IP 11188-RP02
    Anti-B7-H3 Antibody (APC) Mouse Monoclonal FCM 11188-MM06-A
    Anti-B7-H3 Antibody (PE) Mouse Monoclonal FCM 11188-MM06-P
    info info
    添加购物车成功! 添加购物车失败!