Anti-B7-H3 Antibody

Cat: 11188-RP02
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Anti-B7-H3 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-B7-H3 Antibody
Validated applications
ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human B7-H3
Immunogen
Recombinant Human B7-H3 / CD276 protein (Catalog#11188-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human B7-H3 / CD276 (rh B7-H3 / CD276; Catalog#11188-H08H; Q5ZPR3-1; Met 1-Thr 461). B7-H3 / CD276 specific IgG was purified by Human B7-H3 / CD276 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-B7-H3 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-B7-H3 Antibody (Rabbit Polyclonal antibody) Images
Human B7-H3/CD276 Immunoprecipitation(IP) 8063

B7H3 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

2 µL anti-B7H3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-B7H3 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 57 kDa

Observed band size: 110 kDa

Anti-B7-H3 Antibody Alternative Names
Anti-4Ig-B7-H3 Antibody;Anti-B7-H3 Antibody;Anti-B7H3 Antibody;Anti-B7RP-2 Antibody
B7-H3 Background Information

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: ICC Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD276 molecule
References
  • Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
  • Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
  • Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
  • Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
  • Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-B7-H3 Antibody Rabbit Polyclonal ELISA 80380-RP01
    Anti-B7-H3 Antibody Rabbit Polyclonal WB,ELISA,IP 80380-T52
    Anti-B7-H3 Antibody Rabbit Polyclonal ELISA 50973-RP01
    Anti-B7-H3 Antibody Rabbit Polyclonal WB,ELISA,IP 50973-T52
    Anti-B7-H3 Antibody Mouse Monoclonal ELISA 11188-M008
    Anti-B7-H3 Antibody Mouse Monoclonal ELISA(Cap) 11188-MM01
    Anti-B7-H3 Antibody (FITC) Mouse Monoclonal FCM 11188-MM06-F
    Anti-B7-H3 Antibody Rabbit Polyclonal ELISA,IHC-P 11188-T24
    Anti-B7-H3 Antibody Mouse Monoclonal ELISA,ELISA(Det),FCM,ICC/IF,IF 11188-MM06
    Anti-B7-H3 Antibody (APC) Mouse Monoclonal FCM 11188-MM06-A
    Anti-B7-H3 Antibody (PE) Mouse Monoclonal FCM 11188-MM06-P
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