|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
B7H3 was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate
Lane B:0.5 mg 293T Whole Cell Lysate2 µL anti-B7H3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-B7H3 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 57 kDa
Observed band size: 110 kDa
B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: ICC Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-B7-H3 Antibody (FITC)||Mouse||Monoclonal||FCM||11188-MM06-F|
|Anti-B7-H3 Antibody (APC)||Mouse||Monoclonal||FCM||11188-MM06-A|
|Anti-B7-H3 Antibody (PE)||Mouse||Monoclonal||FCM||11188-MM06-P|