Anti-PD-L2 Antibody

Cat: 10292-R018
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Anti-PD-L2 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-PD-L2 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human PD-L2
Has cross-reactivity in ELISA with Human B7-H2 / ICOSL / CD275 Mouse CD86 / B7-2 / B70 Mouse B7-H1 / CD274 / PD-L1
No cross-reactivity in ELISA with Human CD80 / B7-1 Human CD86 / B7-2 / B70 Mouse B7-H2 / ICOSL / CD275
Immunogen
Recombinant Human PD-L2 protein (Catalog#10292-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human PD-L2 / CD273 (rh PD-L2; Catalog#10292-H08H; NP_079515.2; Met 1-Pro 219).
Source
Monoclonal Rabbit IgG Clone #18
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PD-L2 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PD-L2 Antibody (Rabbit Monoclonal antibody) Images
Human PD-L2/B7-DC/CD273 Western blot (WB) 13169

Anti-PD-L2 rabbit monoclonal antibody at 1:500 dilution

Lane A: Raji Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:45 kDa

(We are unsure as to the identity of these extra bands.)

Anti-PD-L2 Antibody Alternative Names
Anti-B7-DC Antibody;Anti-B7DC Antibody;Anti-bA574F11.2 Antibody;Anti-Btdc Antibody;Anti-CD273 Antibody;Anti-PD-L2 Antibody;Anti-PDCD1L2 Antibody;Anti-PDL2 Antibody
PD-L2 Background Information

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
programmed cell death 1 ligand 2
References
  • Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.
  • Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.
  • Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PD-L2 Antibody Rabbit Polyclonal ELISA 50804-T16
    Anti-PD-L2 Antibody Rabbit Monoclonal ELISA 50804-R208
    Anti-PD-L2 Antibody Rabbit Polyclonal ELISA 50804-RP01
    Anti-PD-L2 Antibody Rabbit Polyclonal ELISA 10292-RP02
    Anti-PD-L2 Antibody Rabbit Polyclonal ELISA,IHC-P 10292-T24
    Anti-PD-L2 Antibody Rabbit Polyclonal ELISA 10292-RP01
    Anti-PD-L2 Antibody Rabbit Monoclonal FCM 10292-R002
    Anti-PD-L2 Antibody Mouse Monoclonal ELISA(Cap) 10292-M324
    Anti-PD-L2 Antibody (FITC) Rabbit Monoclonal FCM 10292-R002-F
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