Mouse B4GALT1 Gene cDNA Clone / ORF Clone

B4GALT1 cDNA Clone Datasheet

B4GALT1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_022305.3
• cDNA Size: 1200
• Gene Synonym: GalT; Ggtb; Ggtb2; AA407245; B-1,4-GalT; B4galt1
• Source: Mouse

B4GALT1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

B4GALT1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

B4GALT1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

B4GALT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGTTTC GTGAGCAGTT CCTGGGCGGC AGCGCCGCGA TGCCGGGCGC GACCCTGCAG
61       CGGGCCTGCC GCCTGCTCGT GGCCGTCTGC GCGCTGCACC TCGGCGTCAC CCTCGTCTAT
121      TACCTCTCTG GCCGCGATCT GAGCCGCCTG CCCCAGTTGG TCGGAGTCTC CTCTACACTG
181      CAGGGCGGCA CGAACGGCGC CGCAGCCAGC AAGCAGCCCC CAGGAGAGCA GCGGCCGCGG
241      GGTGCGCGGC CGCCGCCTCC TTTAGGCGTC TCCCCGAAGC CTCGCCCGGG TCTCGACTCC
301      AGCCCTGGTG CAGCTTCTGG CCCCGGCTTG AAGAGCAACT TGTCTTCGTT GCCAGTGCCC
361      ACCACCACTG GACTGTTGTC GCTGCCAGCT TGCCCTGAGG AGTCCCCGCT GCTCGTTGGC
421      CCCATGCTGA TTGACTTTAA TATTGCTGTG GATCTGGAGC TTTTGGCAAA GAAGAACCCA
481      GAGATAAAGA CGGGCGGCCG TTACTCCCCC AAGGACTGTG TCTCTCCTCA CAAGGTGGCC
541      ATCATCATCC CATTCCGTAA CCGGCAGGAG CATCTCAAAT ACTGGCTGTA TTATTTGCAT
601      CCCATCCTTC AGCGCCAGCA ACTCGACTAT GGCATCTACG TCATCAATCA GGCTGGAGAC
661      ACCATGTTCA ATCGAGCTAA GCTGCTCAAT ATTGGCTTTC AAGAGGCCTT GAAGGACTAT
721      GATTACAACT GCTTTGTGTT CAGTGATGTG GACCTCATTC CGATGGACGA CCGTAATGCC
781      TACAGGTGTT TTTCGCAGCC ACGGCACATT TCTGTTGCAA TGGACAAGTT CGGGTTTAGC
841      CTGCCATATG TTCAGTATTT TGGAGGTGTC TCTGCTCTCA GTAAACAACA GTTTCTTGCC
901      ATCAATGGAT TCCCTAATAA TTATTGGGGT TGGGGAGGAG AAGATGACGA CATTTTTAAC
961      AGATTAGTTC ATAAAGGCAT GTCTATATCA CGTCCAAATG CTGTAGTAGG GAGGTGTCGA
1021     ATGATCCGGC ATTCAAGAGA CAAGAAAAAT GAGCCCAATC CTCAGAGGTT TGACCGGATC
1081     GCACATACAA AGGAAACGAT GCGCTTCGAT GGTTTGAACT CACTTACCTA CAAGGTGTTG
1141     GATGTACAGA GATACCCGTT ATATACCCAA ATCACAGTGG ACATCGGGAC ACCGAGATAG

 

Quality Control:cDNA sequencing results is available upon request.