+86-400-890-9989
Product Catalog
B4GALT1 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK11220 |
|
YES | 
|
    |
Human B4GALT1 ELISA Pair Set
 |
Order or Inquire for B4GALT1 ELISA Pair Set |
|
|
High affinity Human B4GALT1 ELISA Pair Set |
|
|
Detection limit - 156 pg/ml |
|
|
Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
|
|
|
|
|
No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
|
Substrate solution was not added |
Add substrate solution and continue |
|
|
Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
|
|
Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
|
Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
|
|
Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
|
|
Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
|
|
Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
|
Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
|
|
High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
|
Increase cycles of washes and soaking time between washes |
||
|
TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
|
|
Materials were contaminated |
Use clean plates, tubes and pipettes tips |
|
|
Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
|
The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human B4GALT1 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Beta-1,4-galactosyltransferase 1, also known as B4GALT1, GGTB2 and beta4GalT1, is a single-pass type I I membrane protein. B4GALT1 is a member of the glycosyltransferase 7 family. It is ubiquitously expressed but at very low levels in fetal and adult brain. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 is an enzyme that functions in the synthesis of glycoconjugates It has two flexible loops, one short and one long. Upon binding a metal ion and UDP-galactose, the loops change from an open to a closed conformation, repositioning residues to lock the ligands in place. B4GALT1 is one of the best-studied glycosyltransferases. It plays a key role in the synthesis of selectin ligands. B4GALT1 may play an important role in the inflammation reaction. Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D). CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation.
