Anti-B4GALT1 Antibody

Cat: 11220-R001
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Anti-B4GALT1 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-B4GALT1 Antibody
Validated applications
ELISA,ELISA(Cap)
Species reactivity
Reacts with: Human
Specificity
Human B4GALT1
Immunogen
Recombinant Human B4GALT1 protein (Catalog#11220-H07H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human B4GALT1 / GGTB2 (rh B4GALT1; Catalog#11220-H07H; Gly 44-Ser 398; NP_001488.2) .
Source
Monoclonal Rabbit IgG Clone #001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-B4GALT1 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:5000-1:10000  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human B4GALT1 / GGTB2 in ELISA pair set (Catalog: # SEK11220). In a sandwich ELISA, it can be used as capture antibody.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-B4GALT1 Antibody Alternative Names
Anti-B4GAL-T1 Antibody;Anti-beta4Gal-T1 Antibody;Anti-CDG2D Antibody;Anti-GGTB2 Antibody;Anti-GT1 Antibody;Anti-GTB Antibody
B4GALT1 Background Information

Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.

Full Name
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
References
  • Hennet T. (2002) The galactosyltransferase family. Cellular and Molecular Life Sciences. 59(7): 1081-95.
  • Landers EA, et al. (2009) Porcine 1, 4-Galactosyltransferase-I Sequence and Expression. Reproduction in Domestic Animals. 44(2): 228-34.
  • Amado M, et al. (2000) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1473 (1): 35-53.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-B4GALT1 Antibody Rabbit Polyclonal ELISA 11220-RP02
    Anti-B4GALT1 Antibody Mouse Monoclonal ELISA 11220-MM02
    Anti-B4GALT1 Antibody Mouse Monoclonal ELISA 11220-MM01
    Anti-B4GALT1 Antibody Rabbit Polyclonal ELISA 11220-RP01
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