Human B3GNT5 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13537-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

B3GNT5 cDNA Clone Datasheet

B3GNT5 cDNA Clone Product Information

Gene Bank Ref.ID:	BC028058
cDNA Size:	1137
Gene Synonym:	B3GN-T5;beta3Gn-T5;B3GNT5
Source:	Human

B3GNT5 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltr DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

B3GNT5 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

B3GNT5 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

B3GNT5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAATGT TGGTTAGTGG CAGAAGAGTC AAAAAATGGC AGTTAATTAT TCAGTTATTT
61       GCTACTTGTT TTTTAGCGAG CCTCATGTTT TTTTGGGAAC CAATCGATAA TCACATTGTG
121      AGCCATATGA AGTCATATTC TTACAGATAC CTCATAAATA GCTATGACTT TGTGAATGAT
181      ACCCTGTCTC TTAAGCACAC CTCAGCGGGG CCTCGCTACC AATACTTGAT TAACCACAAG
241      GAAAAGTGTC AAGCTCAAGA CGTCCTCCTT TTACTGTTTG TAAAAACTGC TCCTGAAAAC
301      TATGATCGAC GTTCCGGAAT TAGAAGGACG TGGGGCAATG AAAATTATGT TCGGTCTCAG
361      CTGAATGCCA ACATCAAAAC TCTGTTTGCC TTAGGAACTC CTAATCCACT GGAGGGAGAA
421      GAACTACAAA GAAAACTGGC TTGGGAAGAT CAAAGGTACA ATGATATAAT TCAGCAAGAC
481      TTTGTTGATT CTTTCTACAA TCTTACTCTG AAATTACTTA TGCAGTTCAG TTGGGCAAAT
541      ACCTATTGTC CACATGCCAA ATTTCTTATG ACTGCTGATG ATGACATATT TATTCACATG
601      CCAAATCTGA TTGAGTACCT TCAAAGTTTA GAACAAATTG GTGTTCAAGA CTTTTGGATT
661      GGTCGTGTTC ATCGTGGTGC CCCTCCCATT AGAGATAAAA GCAGCAAATA CTACGTGTCC
721      TATGAAATGT ACCAGTGGCC AGCTTACCCT GACTACACAG CCGGAGCTGC CTATGTAATC
781      TCCGGTGATG TAGCTGCCAA AGTCTATGAG GCATCACAGA CACTAAATTC AAGTCTTTAC
841      ATAGACGATG TGTTCATGGG CCTCTGTGCC AATAAAATAG GGATAGTACC GCAGGACCAT
901      GTGTTTTTTT CTGGAGAGGG TAAAACTCCT TATCATCCCT GCATCTATGA AAAAATGATG
961      ACATCTCATG GACACTTAGA AGATCTCCAG GACCTTTGGA AGAATGCTAC AGATCCTAAA
1021     GTAAAAACCA TTTCCAAAGG TTTTTTTGGT CAAATATACT GCAGATTAAT GAAGATAATT
1081     CTCCTTTGTA AAATTAGCTA TGTGGACACA TACCCTTGTA GGGCTGCGTT TATCTAA

Quality Control:cDNA sequencing results is available upon request.