Rat B2M Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80423-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

B2M cDNA Clone Datasheet

B2M cDNA Clone Product Information

Gene Bank Ref.ID:	NM_012512.2
cDNA Size:	360
Gene Synonym:	B2m
Source:	Rat

B2M cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus beta-2 microglobulin DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 162 A/G not causing the amino acid variation.

B2M cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

B2M cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

B2M cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCGCT CGGTGACCGT GATCTTTCTG GTGCTTGTCT CTCTGGCCGT CGTGCTTGCC
61       ATTCAGAAAA CTCCCCAAAT TCAAGTGTAC TCTCGCCATC CACCGGAGAA TGGGAAGCCC
121      AACTTCCTCA ACTGCTACGT GTCTCAGTTC CACCCACCTC AGATAGAAAT TGAGCTACTG
181      AAGAATGGAA AGAAGATACC AAATATCGAG ATGTCAGATC TGTCCTTCAG CAAGGACTGG
241      TCTTTCTACA TCCTGGCTCA CACTGAATTC ACACCCACCG AGACCGATGT ATATGCTTGC
301      AGAGTTAAAC ACGTCACTCT GAAGGAGCCC AAAACCGTCA CCTGGGACCG AGACATGTAA

Quality Control:cDNA sequencing results is available upon request.