|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human B2M ORF mammalian expression plasmid, C-GFPSpark tag||HG11976-ACG|
|Human B2M ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11976-ACR|
|Human B2M ORF mammalian expression plasmid, C-Flag tag||HG11976-CF|
|Human B2M ORF mammalian expression plasmid, C-His tag||HG11976-CH|
|Human B2M ORF mammalian expression plasmid, C-Myc tag||HG11976-CM|
|Human B2M ORF mammalian expression plasmid, C-HA tag||HG11976-CY|
|Human B2M Gene cDNA clone plasmid||HG11976-G|
|Human B2M ORF mammalian expression plasmid, N-Flag tag||HG11976-NF|
|Human B2M ORF mammalian expression plasmid, N-His tag||HG11976-NH|
|Human B2M ORF mammalian expression plasmid, N-Myc tag||HG11976-NM|
|Human B2M ORF mammalian expression plasmid, N-HA tag||HG11976-NY|
|Human B2M natural ORF mammalian expression plasmid||HG11976-UT|
|Learn more about expression Vectors|
B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to theα3 chain. Unlikeα3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M(β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(β2 microglobulin) can indicate non-progression of HIV.