>Human cDNA Clones
>Human B2M ORF Expression Clone, FLAG-tagged
Human B2M ORF Expression Clone, FLAG-tagged
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B2M cDNA Clone Datasheet
B2M cDNA Clone Product Information
Gene Bank Ref.ID:
B2M cDNA Clone Description
ORF Clone of Homo sapiens beta-2-microglobulin DNA.
|FLAG Tag Sequence:||GATTACAAGGATGACGACGATAAG|
|Sequence Description:||Identical with the Gene Bank Ref.ID sequence.|
B2M cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
B2M cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
B2M cDNA Clone Sequence and Quality Control
1 ATGTCTCGCT CCGTGGCCTT AGCTGTGCTC GCGCTACTCT CTCTTTCTGG CCTGGAGGCT 61 ATCCAGCGTA CTCCAAAGAT TCAGGTTTAC TCACGTCATC CAGCAGAGAA TGGAAAGTCA 121 AATTTCCTGA ATTGCTATGT GTCTGGGTTT CATCCATCCG ACATTGAAGT TGACTTACTG 181 AAGAATGGAG AGAGAATTGA AAAAGTGGAG CATTCAGACT TGTCTTTCAG CAAGGACTGG 241 TCTTTCTATC TCTTGTACTA CACTGAATTC ACCCCCACTG AAAAAGATGA GTATGCCTGC 301 CGTGTGAACC ATGTGACTTT GTCACAGCCC AAGATAGTTA AGTGGGATCG AGACATGGCT1 361 GATTACAAGG ATGACGACGA TAAG2TAA
1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag
Quality Control:cDNA sequencing results is available upon request.