Human B2M ORF Expression Clone, FLAG-tagged

B2M cDNA Clone Datasheet

B2M cDNA Clone Product Information

• Gene Bank Ref.ID: NM_004048
• cDNA Size: 387
• Gene Synonym: B2M
• Source: Human

B2M cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens beta-2-microglobulin DNA.
• Vector: pCMV/hygro-FLAG
• FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

B2M cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

B2M cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

B2M cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTCGCT CCGTGGCCTT AGCTGTGCTC GCGCTACTCT CTCTTTCTGG CCTGGAGGCT
61       ATCCAGCGTA CTCCAAAGAT TCAGGTTTAC TCACGTCATC CAGCAGAGAA TGGAAAGTCA
121      AATTTCCTGA ATTGCTATGT GTCTGGGTTT CATCCATCCG ACATTGAAGT TGACTTACTG
181      AAGAATGGAG AGAGAATTGA AAAAGTGGAG CATTCAGACT TGTCTTTCAG CAAGGACTGG
241      TCTTTCTATC TCTTGTACTA CACTGAATTC ACCCCCACTG AAAAAGATGA GTATGCCTGC
301      CGTGTGAACC ATGTGACTTT GTCACAGCCC AAGATAGTTA AGTGGGATCG AGACATGGCT1
361      GATTACAAGG ATGACGACGA TAAG2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

 

 

Quality Control:cDNA sequencing results is available upon request.