Mouse B2M Gene cDNA Clone / ORF Clone

B2M cDNA Clone Datasheet

B2M cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009735.3
• cDNA Size: 360
• Gene Synonym: Ly-m11; beta2m; beta2-m; B2m
• Source: Mouse

B2M cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus beta-2 microglobulin DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations:314C/A resulting in the amino acid ala substitution by asp, 52 C/T not causing the amino acid variation.

B2M cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

B2M cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

B2M cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCGCT CGGTGACCCT GGTCTTTCTG GTGCTTGTCT CACTGACCGG CTTGTATGCT
61       ATCCAGAAAA CCCCTCAAAT TCAAGTATAC TCACGCCACC CACCGGAGAA TGGGAAGCCG
121      AACATACTGA ACTGCTACGT AACACAGTTC CACCCGCCTC ACATTGAAAT CCAAATGCTG
181      AAGAACGGGA AAAAAATTCC TAAAGTAGAG ATGTCAGATA TGTCCTTCAG CAAGGACTGG
241      TCTTTCTATA TCCTGGCTCA CACTGAATTC ACCCCCACTG AGACTGATAC ATACGCCTGC
301      AGAGTTAAGC ATGACAGTAT GGCCGAGCCC AAGACCGTCT ACTGGGATCG AGACATGTGA

 

Quality Control:cDNA sequencing results is available upon request.