Autotaxin / ENPP2 Antibody (Antigen Affinity Purified)

Autotaxin / ENPP2 Antibody ( Antigen Affinity Purified ) Datasheet

Autotaxin / ENPP2 Antibody Product Information

• Immunogen: Recombinant Mouse Autotaxin / ENPP2 protein (Catalog#50663-M07H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse Autotaxin / ENPP2 (rM Autotaxin / ENPP2; Catalog#50663-M07H; Q9R1E6-1; Ser 49-Ile 862). Autotaxin / ENPP2 specific IgG was purified by Mouse Autotaxin / ENPP2 affinity chromatography.

Autotaxin / ENPP2 Antibody Usage Guide

• Specificity: Mouse Autotaxin / ENPP2
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ENPP2 in WB. Using a DAB detection system, the detection limit for Mouse ENPP2 is approximately 4 ng/lane under non-reducing conditions and 16 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ENPP2. The detection limit for Mouse ENPP2 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Autotaxin / ENPP2 Antibody Background

Autotaxin (ATX) also known as ectonucleotide pyrophosphatase / phosphodiesterase family member 2 (NPP2 or ENPP2). It belongs to the nucleotide pyrophosphatase /phosphodiesterase family. As a catalytic protein, Autotaxin possesses lysophospholipase D activity, and thus involved in cellular membrane lipid metabolism and remodeling. Autotaxin is discovered in human melanoma cells, has been gaining attention because it could be involved in cancer invasion and metastasis as an autocrine motility factor. Recent evidence has indicated that ATX is a key enzyme in the synthesis of lysophosphatidic acid (LPA), a lipid mediator with a wide range of biological actions including the stimulation of proliferation and contraction in hepatic stellate cells, a pivotal player in hepatic fibrosis. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. Thus, Autotaxin involves in several motility-related processes such as angiogenesis and neurite outgrowth. It was found acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Additionally, Autotaxin / ENPP2 was thought as a culprit protein for cancer, which potently stimulates cancer cell proliferation and tumor cell motility, augments the tumorigenicity and induces angiogenic responses.

References

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4. Tania M, et al., 2010, Biochem Biophys Res Commun. 401 (4): 493-7.