Mouse Art4 / CD297 Protein

ADP-ribosyltransferase 4 Protein Datasheet

Art4 / CD297 Protein Product Information

• Synonym: Art4
• Protein Construction: A DNA sequence encoding the mouse Art4 (NP_080915.1) (Met1-Lys263) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

Art4 / CD297 Protein QC Testing

• Purity: > 91 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 24
• Molecular Mass: The recombinant mouse Art4 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 481 amino acids and has a predicted molecular mass of 53.7 kDa. The apparent molecular mass of the protein is approximately 70 Kda in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Art4 / CD297 protein

Art4 / CD297 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Art4 / CD297 Protein Description

ADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, belongs to a family of ectoenzymes named toxin-related ADP-ribosyltransferases (ARTs) that catalyze the transfer of ADP-ribose from NAD+ to a target protein. ART4 contains a mono-ADP-ribosylation motif. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.

References

1. Parusel I. et al., 2005, Cell Immunol. 236 (1-2): 59-65.
2. Friedrich M. et al., 2005, DNA Seq. 16 (1): 53-7.
3. Okazaki IJ. et al., 1998, J Biol Chem. 273 (37): 23617-20.