Mouse APOH/B2G1 Gene cDNA Clone / ORF Clone

APOH cDNA Clone Datasheet

APOH cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013475.4
• cDNA Size: 1038
• Gene Synonym: B2GPI; beta2-GPI; beta-2-GPI; Apoh
• Source: Mouse

APOH cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus apolipoprotein H DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

APOH cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

APOH cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

APOH cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTTTCCC CGGTGCTCGC CTTGTTCTCC GCCTTCCTCT GCCATGTTGC TATTGCAGGA
61       CGGATCTGTC CGAAGCCGGA TGACCTACCA TTTGCTACGG TTGTCCCCTT AAAGACATCC
121      TACGACCCTG GGGAGCAGAT TGTCTACTCC TGCAAGCCAG GCTACGTGTC CAGGGGAGGG
181      ATGAGACGGT TTACCTGTCC TCTCACAGGA ATGTGGCCCA TCAACACCCT GAGATGTGTC
241      CCCAGAGTAT GTCCTTTCGC TGGAATCTTA GAAAATGGAA TTGTACGCTA CACGAGTTTT
301      GAATATCCCA AGAACATCAG TTTTGCTTGT AACCCTGGGT TTTTTCTGAA TGGGACCAGC
361      TCATCTAAGT GCACGGAGGA AGGAAAATGG AGCCCAGATA TTCCTGCTTG TGCTCGCATC
421      ACCTGCCCGC CACCACCAGT TCCAAAGTTT GCACTCCTTA AGGATTATAG GCCTTCAGCT
481      GGGAACAACT CTTTGTATCA GGACACAGTG GTCTTTAAAT GCTTGCCACA CTTTGCCATG
541      ATCGGAAATG ACACAGTCAT GTGCACAGAA CAAGGAAACT GGACCCGATT GCCAGAATGC
601      CTGGAAGTAA AATGTCCCTT CCCTCCGAGG CCAGAAAATG GGTATGTGAA TTATCCTGCA
661      AAGCCGGTGC TTCTATATAA GGATAAAGCC ACATTTGGTT GCCATGAGAC ATACAAGCTG
721      GACGGCCCAG AAGAAGCGGA ATGTACCAAG ACGGGAACTT GGTCCTTCTT GCCGACCTGT
781      AGAGAGTCTT GCAAACTCCC CGTTAAGAAA GCCACCGTGC TGTACCAAGG GATGAGGGTG
841      AAGATCCAGG AACAGTTTAA GAATGGGATG ATGCATGGCG ACAAAATTCA CTTCTACTGC
901      AAAAACAAAG AGAAGAAGTG CAGCTACACT GTGGAGGCTC ATTGCAGAGA TGGCACTATC
961      GAGATTCCCT CGTGCTTCAA GGAGCACAGT TCTCTGGCTT TCTGGAAAAC GGATGCATCA
1021     GAACTGACAC CGTGCTGA

 

Quality Control:cDNA sequencing results is available upon request.