Mouse ApoAI Gene cDNA Clone / ORF Clone

ApoAI cDNA Clone Datasheet

ApoAI cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009692.3
• cDNA Size: 795
• Gene Synonym: Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; MGC102525; Apoa1
• Source: Mouse

ApoAI cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus apolipoprotein A-I DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations: 745 C/A resulting in the amino acid Gln substitution by lys, 749 T/C resulting in the amino acid val substitution by ala not causing the amino acid variation.

ApoAI cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ApoAI cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ApoAI cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAGCTG TGGTGCTGGC CGTGGCTCTG GTCTTCCTGA CAGGGAGCCA GGCTTGGCAC
61       GTATGGCAGC AAGATGAACC CCAGTCCCAA TGGGACAAAG TGAAGGATTT CGCTAATGTG
121      TATGTGGATG CGGTCAAAGA CAGCGGCAGA GACTATGTGT CCCAGTTTGA ATCCTCCTCC
181      TTGGGCCAAC AGCTGAACCT GAATCTCCTG GAAAACTGGG ACACTCTGGG TTCAACCGTT
241      AGTCAGCTGC AGGAACGGCT GGGCCCATTG ACTCGGGACT TCTGGGATAA CCTGGAGAAA
301      GAAACAGATT GGGTGAGACA GGAGATGAAC AAGGACCTAG AGGAAGTGAA ACAGAAGGTG
361      CAGCCCTACC TGGACGAATT CCAGAAGAAA TGGAAAGAGG ATGTGGAGCT CTACCGCCAG
421      AAGGTGGCGC CTCTGGGCGC CGAGCTGCAG GAGAGCGCGC GCCAGAAGCT GCAGGAGCTG
481      CAAGGGAGAC TGTCCCCTGT GGCTGAGGAA TTTCGCGACC GCATGCGCAC ACACGTAGAC
541      TCTCTGCGCA CACAGCTAGC GCCCCACAGC GAACAGATGC GCGAGAGCCT GGCCCAGCGC
601      CTGGCTGAGC TCAAGAGCAA CCCTACCTTG AACGAGTACC ACACCAGGGC CAAAACCCAC
661      CTGAAGACAC TTGGCGAGAA AGCCAGACCT GCGCTGGAGG ACCTGCGCCA TAGTCTGATG
721      CCCATGCTGG AGACGCTTAA GACCAAAGCC CAGAGTGTGA TCGACAAGGC CAGCGAGACT
781      CTGACTGCCC AGTGA

 

Quality Control:cDNA sequencing results is available upon request.