Mouse ApoA1 Protein (His Tag)

Apolipoprotein A-I Protein Datasheet

ApoA1 Protein Product Information

• Synonym: ApoA1
• Protein Construction: A DNA sequence encoding the mouse ApoA1 (Q00623) (Met1- Gln264) was expressed with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

ApoA1 Protein QC Testing

• Purity: >94% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Trp 19
• Molecular Mass: The recombinant mouse ApoA1 comprises 257 amino acids and has a predicted molecular mass of 30.2 kDa. The apparent molecular mass of the protein is approximately 27-31 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ApoA1 protein

ApoA1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ApoA1 Protein Description

Apolipoprotein A1 (APOA1) is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase(LCAT) which is responsible for the formation of most plasma cholesteryl esters. and thus play a major role in cholesterol efflux from peripheral cells. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. Men carrying a mutation may develop premature coronary artery disease.

References

1. Toptas B. et al., 2011, In Vivo. 25 (3): 425-30.
2. Haase CL. et al., 2011, J Intern Med. 270 (2): 136-46.
3. Wu Z. et al., 2011, J Biol Chem. 286 (14): 12495-508.