Antibody purification protein

Sino Biological Purification Agarose
  • Higher Binding Capacity
  • Higher Bead Rigidity
  • Buy in bulk, Save even more

•   Protein A for Antibody purification  learn more...

Species

Staphylococcal

Purity

> 95% by SDS-PAGE

Expression System

E. coli

Molecular Mass

34kD

Form

Liquid or lyophilized

Size / Price

10mg ($50)
1g ($480)
10g ($4000)
Bulk

•   Protein G for Antibody purification  learn more...

Species

Staphylococcal

Purity

> 95% by SDS-PAGE

Expression System

E. coli

Molecular Mass

31kD

Form

Liquid or lyophilized

Size / Price

1mg ($50)
5mg ($160)
10mg ($270)
Bulk

•   Protein L for Antibody purification  learn more...

Species

Peptostreptococcus magnus

Purity

> 95% by SDS-PAGE

Expression System

E. coli

Molecular Mass

39kD

Form

Liquid or lyophilized

Size / Price

1mg ($80)
5mg ($260)
10mg ($420)
Bulk

 

Antibody Purification

 Antibody Purification Tools

   Antibody Purification Agarose

  •  Protein A agarose beads (resin) for Antibody purification

  •  Protein G agarose beads (resin) for Antibody purification

  •  Protein L agarose beads (resin) for Antibody purification

    Antibody Purification Protein

  •  Protein A for Antibody purification

  •  Protein G for Antibody purification

  •  Protein L for Antibody purification

 Antibody Purification Technologies & Methods

   Antigen Affinity Purification

   Affinity Purification Principle

  •  Affinity Purification Principle by Protein A

  •  Affinity Purification Principle by Protein L

  •  Affinity Purification Principle by Protein G

   Antibody Concentration Methods

  •  Antibody Concentration Methods by Crystallization

  •  Antibody Concentration Methods by Precipitation

  •  Antibody Concentration Methods by Lyophilization

  •  Antibody Concentration Methods by Ultrafiltration

   Antibody Purification Methods

   Antibody Polish Methods

  •  Antibody Polish Methods by Hydrophobic Interaction Chromatography

  •  Antibody Polish Methods by Ion Exchange Chromatography

  •  Antibody Polish Methods by Size Exclusion Chromatography

 

 Related Links:

   Protein L/G/A Characteristics and IgG Binding Properties

  •  Protein L Characteristics and IgG Binding Properties

  •  Protein G Characteristics and IgG Binding Properties

  •  Protein A Characteristics and IgG Binding Properties

   Services

  •  Antibody Production Services

  •  Antibody Development Services

  •  Protein Production Services

Protein A, protein G, and Protein L are native and recombinant proteins of microbial origin that bind to mammalian immunoglobulins (Ig). Protein A and protein G bind with high affinity and specificity to the Fc region of immunoglobulins from various mammalian species, most notably the IgGs. Protein L binds immunoglobulins via the kappa light chain. These antibody-binding proteins can be used to purify, immobilize or detect immunoglobulins. These proteins (i.e. protein A, protein G, protein L) are typically attached onto a solid support such as sepharose or magnetic beads. Table 1 summarizes the various formats available for the antibody-binding proteins.

Antibodies are a particularly important class of complex proteins that are increasingly used as immunochemical tools in research and for diagnostic and therapeutic use. Streptococcal Protein A and Protein G are well-established high affinity ligands for capture of IgG antibodies. These proteins bind to the constant (Fc) region of IgG molecules from a variety of species, but differ in selectivity for species and subclass of IgG. Most intact IgG antibodies can be purified efficiently by affinity chromatography on Protein A or Protein G media. However, antibody fragments such as Fab or single-chain Fv (scFv) fragments as well as other antibody classes (IgA, IgE, IgM, etc.) may require a different approach. Selection of monoclonal antibodies by binding to the Fc region can give single-specificity preparations, but antibodies selected from serum will typically be directed towards a broad range of antigens. Customized affinity chromatography media carrying antigen as ligand can be used for selection of single-specificity antibodies from such preparations.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"