Anti-human SLAMF1 / CD150 / SLAM antibody

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
10837-MM05	200µg ($95) 500µg ($195)		YES
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Signaling Lymphocytic Activation Molecule Antibody

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		Detection limit is 2 ng/lane in WB
		Detection limit is 0.31 ng/well in ELISA

SLAMF1 / CD150 / SLAM Antibody Product Information

Immunogen :	Recombinant human CD150 protein ( Catalog#10837-H08H )
Antibody Type :	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID :	11B1H1B4
Ig Type :	Mouse IgG1
Formulation :	0.2 μm filtered solution in PBS with 5% trehalose
Preparation :	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human SLAMF1 / CD150 ( rh SLAMF1 ; Catalog#10837-H08H ; NP_003028.1; Met 1 - Pro 258 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

SLAMF1 / CD150 / SLAM Antibody Usage Guide

Specificity :	Human SLAMF1 / CD150 / SLAM
	No cross-reactivity in WB and ELISA with Human CD48 / BCM1 / SLAMF2 Human CD84 / SLAMF5 Human 2B4 / SLAMF4 / CD244 Human cell lysate (293 cell line)
Western blot :	This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human SLAMF1 in WB. Using a DAB detection system, the detection limit for human SLAMF1 is approximately 20 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions
Direct ELISA ：	This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human SLAMF1. The detection limit for human SLAMF1 is 0.31 ng/well
Storage :	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SLAMF1 / CD150 / SLAM Antibody Related Products & Topics

Stem Cell>>Hematopoietic Stem Cell (HSC)>>Hemangioblast>>Endothelial Cell Marker>>CD150/SLAM

Immunology>>Adaptive Immunity>>Costimulation & Costimulatory Molecule>>CD150/SLAM

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>CD150/SLAM

Immunology>>Cluster of Differentiation>>Hematopoietic Stem Cell CD Marker>>CD150/SLAM

Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Macrophage Markers / Monocyte Markers>>CD150/SLAM

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Helper T Cells>> CD150/SLAM

Proteins:

Molecule	Species	Description //For Detailed Info.------CLICK!	Cat No	Size/Price
CD150/SLAM	Human	CD150/SLAM Protein, Recombinant	10837-H08H	100µg($290);Order

Antibodies:

Molecule	Application	Description //For Detailed Info.------CLICK!	Cat No	Size/Price
Human CD150/SLAM	WB, ELISA	Mouse Monoclonal Antibody	10837-MM05	Order
Human CD150/SLAM	WB, ELISA	Rabbit Monoclonal Antibody	10837-R008	Order
Human CD150/SLAM	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10837-RP01	100µg($250); Order
Human CD150/SLAM	WB, ELISA	Rabbit Polyclonal Antibody	10837-RP02	Order

SLAMF1 / CD150 / SLAM Antibody Background

Signaling lymphocytic activation molecule (SLAM) is a cell surface sialylated phospho-glycoprotein and belongs to the CD2 subset of the Ig superfamily of type I transmembrane glycoproteins. It has a 77-residue cytoplasmic tail containing several tyrosines within motifs for Src-homology 2 (SH2)-domain binding sites, and interacts with SH2 domain protein 1A (SH2D1A) and with PTPN11. LAM is constitutively expressed on peripheral-blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. As a self-ligand, CD150 performs diverse immunologic functions including T/B-cell costimulation, induction of interferon γ (IFN-γ) in Th1 T-cell clones, redirection of Th2 clones to a Th1 or Th0 phenotype, and inhibition of apoptosis in B cells. Furthermore, CD150 was shown to be the second receptor for measles virus in addition to CD46, and the distribution of SLAM on various cell lines is consistent with their susceptibility to clinical isolates of measles virus.

References

Svitlana V. Mikhalap, et al., 1999, The Journal of Immunology. 162: 5719-27.
Duncan Howie, et al., 2002, Blood, Vol. 99, No. 3: 957-65.
Benjamin G. Cocks, et al., 1995, Nature. 376: 260-3.
Hironobu Tatsuo, et al., 2000, Nature, 406: 893-7.
Svetlana P. et al., 2003，Nature Immunology, 4: 19-24.
G. Grant Welstead, et al., 2004, Journal of Virology, Vol. 78, No. 18: 9666-74.

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