Anti-human PDGFRa / CD140a antibody
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| 10556-MM03 |
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Alpha-Type Platelet-Derived Growth Factor Receptor Antibody
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Order or Inquire for PDGFRa Antibody product |  |
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Detection limit is 0.0195 ng/well in ELISA |
PDGFRa / CD140a Antibody Product Information
| Immunogen : |
Recombinant human CD140a protein ( Catalog#10556-H08H ) |
| Antibody Type : | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
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Clone ID : |
5A10H9 |
| Ig Type : |
Mouse IgG1 |
| Formulation : | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation : |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human extracellular domain PDGFRa / CD140a ( rh PDGFRa ; Catalog#10556-H08H ; NP_006197.1 ; Met 1- Glu 524 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
PDGFRa / CD140a Antibody Usage Guide
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Specificity : |
Human PDGFRa / CD140a |
| No cross-reactivity in WB and ELISA with Human PDGFRb / CD140b and human cell lysate (293 cell line) | |
| Direct ELISA ： | This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human PDGFRa. The detection limit for human PDGFRa is 0.0195 ng/well |
| Storage : | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
PDGFRa / CD140a Antibody Related Products & Topics
PDGFRa / CD140a Antibody Related Areas:
Proteins:
Antibodies:
PDGFRa / CD140a Antibody Background
PDGFRα, also known as CD140a, together with the structurally homolog protein PDGFRβ (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin -like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRα is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRβ dose not bind PDGF-AA. PDGF binding induces the receptor homo- and hetero-dimerization as well as activation of the receptor, and subsequently activates c-Src tyrosine kinase which relays signals to PI 3 kinase and MAPK cascades. PDGFRα thus plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRα acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development.
References
- Matsui, T. et al., 1989, Science. 243: 800-804.
- Yokote, K. et al., 1996, J. Biol. Chem. 271: 5101-5111.
- Choudhury, GG. et al., 2000, Kidney. Int. 57: 908-917.
- Brennan, J. et al., 2003, Genes & Dev. 17: 800-810.
- Choudhury, GG. et al., 2006, Cell. Signal. 18: 1854-1864.