Anti-human P4HB / ERBA2L antibody
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| 10827-MM08 |
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Prolyl 4-hydroxylase Antibody ( P4HB / ERBA2L )
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Order or Inquire for P4HB Antibody product |  |
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Detection limit is 1 ng/lane in WB | ||
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Detection limit is 0.039 ng/well in ELISA |
P4HB / ERBA2L Antibody Product Information
| Immunogen : |
Recombinant human P4HB protein ( Catalog #10827-H08H ) |
| Antibody Type : | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
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Clone ID : |
2F6G12H2 |
| Ig Type : |
Mouse IgG1 |
| Formulation : | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation : |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human P4HB / ERBA2L ( rh P4HB ; Catalog #10827-H08H ; NP_000909.2 ; Met 1- Lys 505 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
P4HB / ERBA2L Antibody Usage Guide
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Specificity : |
Human P4HB / ERBA2L |
| No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA. | |
| Western blot : | This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human P4HB in WB. Using a DAB detection system, the detection limit for human P4HB is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions |
| Direct ELISA ： | This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human P4HB. The detection limit for human P4HB is 0.039 ng/well |
| Storage : | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
P4HB / ERBA2L Antibody Related Products & Topics
P4HB / ERBA2L Antibody Related Areas:
Proteins:
Antibodies:
P4HB / ERBA2L Antibody Background
Prolyl 4-hydroxylase belongs to the group of 2-oxoglutarate coupled dioxygenases, and catalyzes the formation of 4-hydroxyproline in collagens by the hydroxylation of proline residues in peptide linkages. The reaction product, the genetically uncoded amino acid 4-hydroxyproline, plays a crucial role in collagens, as the hydroxyl groups of these amino acid residues are essential for the folding of the newly synthesized procollagen polypeptide chains into triple-helical molecules. The active enzyme is a tetramer (α2β2), and the β subunit (P4HB) alnoe has been found to be identical to the enzyme protein disulfide isomerase (PDI). P4HB is multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. Inside the cell, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins at high concentrations, while facilitates aggregation (anti-chaperone activity) at low concentrations. In addition to a subunit of polyl 4-hydroxylase, P4HB also acts as a structure subunit of microsomal triacylglycerol transfer protein MTTP.
References
- Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17.
- Pihlajaniemi T, et al., 1991, J Hepatol., 13, Suppl 3: S2.
