Anti-human NTRK2 / Trk-B / TRKB antibody
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| 10047-MM05 |
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Neurotrophic Tyrosine Receptor Kinase 2 ( NTRK2 / Trk-B ) Antibody
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Order or Inquire for Trk-B Antibody product |  |
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Detection limit is 4 ng/lane in WB |
NTRK2 / Trk-B / TrkB Antibody Product Information
| Immunogen : |
Recombinant human Trk-B protein ( Catalog#10047-H08H ) |
| Antibody Type : | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
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Clone ID : |
5F1B11 |
| Ig Type : |
Mouse IgG1 |
| Formulation : | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation : |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human NTRK2 / Trk-B extracellular domain ( rh Trk-B; Catalog#10047-H08H; NP_001007098.1; Met 1- His 430 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography |
NTRK2 / Trk-B / TrkB Antibody Usage Guide
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Specificity : |
Human NTRK2 / Trk-B / TrkB |
| No cross-reactivity in WB and ELISA with Human Trk-A / NTRK1 / TRK Human Trk-C / NTRK3 / TRKC Mouse Trk-C / NTRK3 / TRKC Human cell lysate (293 cell line) |
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| Western blot : | This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human Trk-B in WB. Using a DAB detection system, the detection limit for human Trk-B is approximately 20 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions |
| Storage : | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
NTRK2 / Trk-B / TrkB Antibody Related Products & Topics
NTRK2 / Trk-B / TrkB Antibody Related Areas:
Proteins:
Antibodies:
NTRK2 / Trk-B / TrkB Antibody Background
TrkB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor exclusively expressed in neurons of the central nervous system. BDNF(brain-derived neurotrophic factor) exerts biological actions through its physiological receptor TrkB which leads to the initiation of intracellular signaling cascades and different biological responses in neurons. TRKB has two distinct binding regions for BDNF within the extracellular domain with high affinity. Upon neurotrophin binding, TrkB phosphorylates itself and the downstream molecular members of the related signal pathways. TrkB has been shown to play an important role in tumorigenesis and metastasis, and mutations in this gene are associated with obesity and mood disorders. Accordingly, BDNF/TrkB signaling is thought to be a potential therapeutic target for schizophrenia.
References
- Klein, R. et al., 1990, Cell. 64: 647-56.
- Haniu, M. et al., 1997, J. Biol. Chem. 272 : 25296-303.
- Haapasalo, A. et al., 2002, J. Biol. Chem. 277: 43160-7.
- Pillai, A., 2008, Neurosignals. 16: 183-93.
- Thomas, R. et al., 2007, Cancer. Res. 67: 6221-9.
