Anti-human CD200 / OX-2 antibody
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| 10886-MM03 |
|
YES |  |
    |
|
OX-2 Membrane Glycoprotein ( CD200 / OX-2 ) Antibody
 |
Order or Inquire for CD200 Antibody product |  |
|
|
Detection limit is 4 ng/lane in WB | ||
|
Detection limit is 0.16 ng/well in ELISA |
CD200 / OX-2 Antibody Product Information
| Immunogen : |
Recombinant human CD200 protein ( Catalog#10886-H08H ) |
| Antibody Type : | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
|
Clone ID : |
8E10B7 |
| Ig Type : |
Mouse IgG1 |
| Formulation : | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation : |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human CD200 ( rh CD200 ; Catalog#10886-H08H ; NP_005935.4 ; Met 1- Gly 232 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
CD200 / OX-2 Antibody Usage Guide
|
Specificity : |
Human CD200 / OX-2 |
| No cross-reactivity in WB and ELISA with Mouse CD200 / OX-2 and human cell lysate (293 cell line) | |
| Western blot : | This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CD200 in WB. Using a DAB detection system, the detection limit for human CD200 is approximately 4 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions |
| Direct ELISA ： | This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human CD200. The detection limit for human CD200 is 0.16 ng/well |
| Storage : | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
CD200 / OX-2 Antibody Related Products & Topics
CD200 / OX-2 Antibody Related Areas:
Proteins:
Antibodies:
CD200 / OX-2 Antibody Background
CD200, also known as OX-2, is a transmembrane immunoregulatory protein belonging to the immunoglobulin superfamily. The human CD200 cDNA encodes a 278 aa precursor that includes a 30 aa signal sequence, a 202 aa extracellular domain (ECD), a 27 aa transmembrane segment, and a 19 aa cytoplasmic domain. The ECD is composed of one Ig-like V-type domain and one Ig-like C2-type domain, and shares 76% aa sequence identity with mouse and rat CD200. CD200 interacts with a structurally related receptor (CD200R) expressed mainly on myeloid cells and is involved in regulation of macrophage and mast cell function. CD200 and CD200R associate via their respective N-terminal Ig-like domains. In myeloid cells, CD200R initiates inhibitory signals following receptor-ligand contact. In T cells, CD200 functions as a costimulatory molecule independent of the CD28 pathway. In addition, CD200 also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion.
References
- Yu, J.X. et al., 1994, J. Biol.Chem. 269: 18843-8.
- Chen, L.M., 2001, J. Biol.Chem. 276: 21434-42.
- Chen, L.M. et al., 2002, Int. J. Cancer. 97: 323-329.
- Chen, M. et al., 2007, Biochim. Biophys. Acta. 1733: 1133-40.
- Verghese, G.M. et al., 2006, Am. J. Physiol. Cell. Physiol. 291: c1285-70.
