Anti-V5 tag Antibody

Cat: 100378-MM01
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Anti-V5 tag Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-V5 tag Antibody
Validated applications
WB,IP
Specificity
V5 tag
Immunogen
A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #01
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-V5 tag Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
IP 1-4 μg/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-V5 tag Antibody (Mouse Monoclonal antibody) Images
  Western blot (WB) 14828

Anti-V5 Tag mouse monoclonal antibody at 1:500 dilution.

Lane A: his-ARG1-V5 transfected 293 cell lysate(60ug)

Lane B: mFABP4-V5-his transfected 293 cell lysate(60ug)

Lane C: V5-ARG1-his transfected 293 cell lysate(10ug)

Lane D: V5-mFABP4-his transfected 293 cell lysate(20ug)

Lane E: Negative control transfected 293 cell lysate(30ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

  Immunoprecipitation(IP) 14830

V5 tag was immunoprecipitated using:

Lane A:0.5 mg V5-ARG1-His transfected 293 Whole Cell Lysate

Lane B:0.5 mg V5-mFABP4-His transfected 293 Whole Cell Lysate

2 µL anti-V5 tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-V5 tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: kDa

Observed band size: kDa

Product Description Host Clonality Application Catalog# (PDF)
Anti-V5 tag Antibody Mouse Monoclonal WB 100378-MM03
Anti-V5 tag Antibody Mouse Monoclonal ICC/IF,IF 100378-MM04
Anti-V5 tag Antibody Rabbit Polyclonal WB,IP 100378-T36
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