Anti-V5 Antibody

Cat: 100378-MM04

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Anti-V5 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-V5 Antibody
Validated applications
A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #04
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-V5 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:20-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-V5 Antibody (Mouse Monoclonal antibody) Images
  Immunohistochemistry(IHC) 2739
Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
Product Description Host Clonality Application Catalog# (PDF)
Anti-V5 Antibody Mouse Monoclonal WB,IP 100378-MM01
Anti-V5 Antibody Mouse Monoclonal WB 100378-MM03
Anti-V5 Antibody Rabbit Polyclonal WB,IP 100378-T36
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