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Anti-V5 Tag Antibody, Mouse Mab

  • Anti-V5 Tag Antibody, Mouse MAb, Immunofluorescence
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 V5 Antibody Product Information
Immunogen:A synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST).
Clone ID:04
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the V5 tag sequence (GKPIPNPLLGLDST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 V5 Antibody IF Application Image
Anti-V5 Tag Antibody, Mouse MAb, Immunofluorescence
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Caption:
Immunofluorescence staining of V5-Tag in Hela cells, transfected with pSTEP2-V5-mFABP4-his (Figure A), pSTEP2-mFABP4-V5-his (Figure B), pSTEP2-V5-ARG1-his (Figure C) and pSTEP2-his-ARG1-V5 (Figure D). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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Catalog: 100378-MM04-50
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Datasheet & Documentation

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"