Anti-S-tag Antibody

Cat: 101290-T38
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Anti-S-tag Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-S-tag Antibody
Validated applications
WB,ICC/IF,IF,IP
Specificity
S-tag
Immunogen
A synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS).
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS), and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-S-tag Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:2000-1:10000  
ICC-IF 1:300-1:10000  
IP 0.5-2 μg/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-S-tag Antibody (Rabbit Polyclonal antibody) Images
 S-tag Immunofluorescence(IF) 2751
Immunofluorescence staining of S-Tag in Hela cells, transfected with pSTEP2-Stag-GFERh (Figure A), pSTEP2-his-GFER-Stag (Figure B), pSTEP2-his-Stag-GFER (Figure C), pSTEP2-Stag-GSTT2h (Figure D),pSTEP2-his-Stag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Stag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-S-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
 S-tag Immunoprecipitation(IP) 6010

s-tag was immunoprecipitated using:

Lane A:0.5 mg Stag-GFERh© transfected 293 Whole Cell Lysate

Lane B:0.5 mg his-GFER-Stag(C) transfected 293 Whole Cell Lysate

Lane C:0.5 mg his-Stag-GFER(C) transfected 293 Whole Cell Lysate

Lane D:0.5 mg Psetp2 transfected 293 Whole Cell Lysate

Lane E:0.5 mg his-Stag-GSTT2© Whole Cell Lysate

2 µL anti-s-tag rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-s-tag rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

 S-tag Western blot (WB) 6581

Anti-S Tag rabbit polyclonal antibody at 1:5000 dilution

Lane A: Stag-GFERh(C) transfected 293 cell lysate at 2ug

Lane B: his-GFER-Stag(C) transfected 293 cell lysate at 2ug

Lane C: his-Stag-GFER(C) transfected 293 cell lysate at 2ug

Lane D: Stag-GSTT2h(C) transfected 293 cell lysate at 10ug

Lane E: his-Stag-GSTT2(C) transfected 293 cell lysate at 10ug

Lane F: his-GSTT2-Stag(C) transfected 293 cell lysate at 10ug

Lane G: Nagetive control at 10ug

Secondary

Dylight 800-labeled Antibody To Rabbit IgG (H+L) at 1/5000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Product Description Host Clonality Application Catalog# (PDF)
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