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Anti-S-tag Antibody, Rabbit Pab, Antigen Affinity Purified

  • Anti-S-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Western Blot
  • Anti-S-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
  • Anti-S-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunoprecipitation
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  Antibody Product Information
Immunogen:A synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS).
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS
Preparation:Produced in rabbits immunized with a synthetic peptide corresponding to the S-tag sequence (KETAAAKFERQHMDS), and purified by antigen affinity chromatography.
  Antibody WB Application Image
  Antibody IF Application Image
Anti-S-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunofluorescence staining of S-Tag in Hela cells, transfected with pSTEP2-Stag-GFERh (Figure A), pSTEP2-his-GFER-Stag (Figure B), pSTEP2-his-Stag-GFER (Figure C), pSTEP2-Stag-GSTT2h (Figure D),pSTEP2-his-Stag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Stag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-S-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
  Antibody IP Application Image
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"