MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Antibody, Mouse MAb

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MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal antibody) General Information

Product name
MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Antibody, Mouse MAb
Validated applications
WB,ELISA,IHC-P,FCM,ICC/IF,IP (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
MERS-CoV CoV Nucleoprotein
Immunogen
Recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (Catalog#40068-V08B)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP (Catalog#40068-V08B; AFS88943.1; Met 1-Asp413). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #10
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 1:1000-1:5000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Antibody, Mouse MAb (Mouse Monoclonal antibody) Images

Anti-MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP mouse monoclonal antibody at 1:1000

Sample:MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP

Lane A: 20ng

Lane B: 5ng

Lane C: 2ng

Secondary

Rabbit Anti-Mouse IgGF (ab)2/HRP at 1/10000 dilution.

Performed under reducing conditions.

MERS-CoV (NCoV / Novel coronavirus) Nucleocapsid Antibody, Mouse MAb: Alternative Names

Anti-NP Antibody

CoV Nucleoprotein Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronaviruses primarily cause respiratory and enteric diseases in mammals and birds. Coronaviruses can cause a range of symptoms varying from mild symptoms such as the common cold to more serious respiratory illnesses. They primarily cause respiratory and enteric diseases in mammals and birds. Coronavirus symptoms include rhinorrhea, sneezing, cough, nasal obstruction, bronchitis and so on. There are three main groups of coronaviruses: alpha, beta, and gamma. Proteins that contribute to the overall structure of all coronaviruses are the spike (S), envelope (E), membrane (M) and nucleoprotein (N). Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch.
References
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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