Anti-His tag Antibody (HRP)

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Anti-His tag Antibody (HRP) (Mouse Monoclonal antibody) General Information

Product name
Anti-His tag Antibody (HRP)
Validated applications
WB,ELISA
Specificity
His tag
Immunogen
A synthetic peptide corresponding to 6×His
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP).
Source
Monoclonal
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
HRP
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-His tag Antibody (HRP) (Mouse Monoclonal antibody) Validated Applications

Application Dilution
WB 0.2-1 μg/mL
ELISA 0.1-0.4 μg/mL
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-His tag Antibody (HRP) (Mouse Monoclonal antibody) Images

Anti-His Tag (HRP) mouse monoclonal antibody at 1 μg/mL.

Lane A: Recombiant Human LRG1 (C-terminal His), 50ng

Lane B: Recombiant Human 4-1BB (C-terminal His), 50ng

Developed using the ECL technique.  Performed under reducing conditions.

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