Anti-Her2 / ERBB2 Antibody

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Anti-Her2 / ERBB2 Antibody (Rabbit Monoclonal antibody) General Information

Product name
Anti-Her2 / ERBB2 Antibody
Validated applications
ELISA,IHC-P,FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human Her2 / ERBB2
Immunogen
Recombinant Human HER2/ErbB2/CD340 Protein (Catalog#10004-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human HER2/ErbB2/CD340 (rh HER2/ErbB2/CD340; Catalog#10004-H08H; NP_004439.2; Met1-Thr652).
Source
Monoclonal Rabbit IgG Clone #002
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Her2 / ERBB2 Antibody (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Her2 / ERBB2 Antibody (Rabbit Monoclonal antibody) Images

Flow cytometric Analysis of anti-ErbB2 (10004-R002A, 0.5 μL/test) on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-human ErbB2. Second step staining with FITC conjugated goat anti-rabbit IgG (H+L) polyclonal antibody.
Immunochemical staining of human ERBB2 in human breast carcinoma with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of Erbb2 in SKBR3 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human Erbb2 monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and Cell membrane.

Anti-Her2 / ERBB2 Antibody: Alternative Names

Anti-CD340 Antibody; Anti-ENV Antibody; Anti-ENVW Antibody; Anti-ERVWE1 Antibody; Anti-HER-2 Antibody; Anti-HER-2/neu Antibody; Anti-HER2 Antibody; Anti-HERV-7q Antibody; Anti-HERV-W-ENV Antibody; Anti-HERV7Q Antibody; Anti-HERVW Antibody; Anti-HERVWENV Antibody; Anti-MLN 19 Antibody; Anti-MLN19 Antibody; Anti-NEU Antibody; Anti-NGL Antibody; Anti-TKR1 Antibody

Her2 / ERBB2 Background Information

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
Full Name
erb-b2 receptor tyrosine kinase 2
References
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.

Standard Antibody Development Service

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Rabbit PAb

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Mouse MAb

Rabbit PAb

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