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Anti-HA Tag Antibody, Mouse MAb

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 HA Antibody Product Information
Immunogen:A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).
Clone ID:10
Ig Type:Mouse IgG1
Concentration:0.5 μl/Test
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
 HA Antibody Usage Guide
Specificity:Recognize N-terminal and C-terminal HA Tag in fusion proteins.
Application:WB, ELISA, ICC/IF, FCM, IF,IP

WB: 1/2000-1/5000

ELISA: 1/2000-1/5000

This antibody can be used at 1/2000-1/5000 dilution with the appropriate secondary reagents to detect HA-tagged protein (amino-terminus HA tag or carboxy-terminus HA tag).

FCM: 1/50-1/200

ICC/IF: 1/200-1/500

IP: 2-8 μL/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 HA Antibody ELISA Application Image
[Click to enlarge image]
Caption:
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HA-tagged protein (amino-terminus HA tag or carboxy-terminus HA tag).
Anti-HA Tag Antibody, Mouse MAb, ELISA
 HA Antibody WB Application Image
 HA Antibody IHC Application Image
Anti-HA Tag Antibody, Mouse Mab, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (L) or mock-transfected (R). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with SBI Mouse anti-HA-tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green).
Anti-HA Tag Antibody, Mouse Mab, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D). Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
 HA Antibody FC Application Image
Anti-HA Tag Antibody, Mouse Mab, Flow Cytometry
[Click to enlarge image]
Caption:
Profile of anti-HA Tag reactivity on 293 transfected cells analyzed by flow cytometry. 293 cells transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D) were fixed and permeabilized according to the manufacturer's manual (Cat. No. 554714,BD Pharmingen) and subsequently stained with Purified Mouse Anti-HA Tag antibody (Cat No. 100028-MM10) Or positive control antibody  for 30 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 30 min on ice. Cells were washed twice and analyzed by flow cytometry.
 HA Antibody IP Application Image
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                                                                                                                                                      50 µg 
                                                                                                                                                      100 µg 
                                                                                                                                                      Add to Cart
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                                                                                                                                                        11048-RM09-50
                                                                                                                                                        11048-RM09-100
                                                                                                                                                        50 µg 
                                                                                                                                                        100 µg 
                                                                                                                                                        Add to Cart
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                                                                                                                                                          86001-RM01-50
                                                                                                                                                          86001-RM01-100
                                                                                                                                                          50 µg 
                                                                                                                                                          100 µg 
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                                                                                                                                                            50 µg 
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                                                                                                                                                            Application: ELISA  WB  FCM  
                                                                                                                                                            • Slide 1
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                                                                                                                                                            50 µg 
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                                                                                                                                                              50 µg 
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                                                                                                                                                                11048-MM11-50
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                                                                                                                                                                50 µg 
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                                                                                                                                                                Reactivity: Influenza  
                                                                                                                                                                Application: ELISA  WB  
                                                                                                                                                                  11699-RP01-100
                                                                                                                                                                  11699-RP01-200
                                                                                                                                                                  100 µg 
                                                                                                                                                                  200 µg 
                                                                                                                                                                  Add to Cart
                                                                                                                                                                  Reactivity: Influenza  
                                                                                                                                                                  Application: ELISA  WB  IHC-P  IP  
                                                                                                                                                                    11699-RP02-50
                                                                                                                                                                    11699-RP02-100
                                                                                                                                                                    50 µg 
                                                                                                                                                                    100 µg 
                                                                                                                                                                    Add to Cart
                                                                                                                                                                    Reactivity: Influenza  
                                                                                                                                                                    Application: ELISA  WB  B/N  
                                                                                                                                                                      11048-MM01-50
                                                                                                                                                                      11048-MM01-100
                                                                                                                                                                      50 µg 
                                                                                                                                                                      100 µg 
                                                                                                                                                                      Add to Cart
                                                                                                                                                                      Reactivity: Influenza  
                                                                                                                                                                      Application: ELISA  
                                                                                                                                                                        11048-MM06-50
                                                                                                                                                                        11048-MM06-100
                                                                                                                                                                        50 µg 
                                                                                                                                                                        100 µg 
                                                                                                                                                                        Add to Cart
                                                                                                                                                                        Reactivity: Influenza  
                                                                                                                                                                        Application: ELISA  WB  
                                                                                                                                                                          11048-RM07-50
                                                                                                                                                                          11048-RM07-100
                                                                                                                                                                          50 µg 
                                                                                                                                                                          100 µg 
                                                                                                                                                                          Add to Cart
                                                                                                                                                                          Reactivity: Influenza  
                                                                                                                                                                          Application: ELISA  WB  
                                                                                                                                                                            11696-RP01-100
                                                                                                                                                                            11696-RP01-200
                                                                                                                                                                            100 µg 
                                                                                                                                                                            200 µg 
                                                                                                                                                                            Add to Cart
                                                                                                                                                                            Reactivity: Influenza  
                                                                                                                                                                            Application: ELISA  WB  IHC-P  IP  
                                                                                                                                                                              11696-RP02-50
                                                                                                                                                                              11696-RP02-100
                                                                                                                                                                              50 µg 
                                                                                                                                                                              100 µg 
                                                                                                                                                                              Add to Cart
                                                                                                                                                                              Reactivity: Influenza  
                                                                                                                                                                              Application: ELISA  WB  B/N  
                                                                                                                                                                                11048-MM01-50
                                                                                                                                                                                11048-MM01-100
                                                                                                                                                                                50 µg 
                                                                                                                                                                                100 µg 
                                                                                                                                                                                Add to Cart
                                                                                                                                                                                Reactivity: Influenza  
                                                                                                                                                                                Application: ELISA  
                                                                                                                                                                                  11048-MM06-50
                                                                                                                                                                                  11048-MM06-100
                                                                                                                                                                                  50 µg 
                                                                                                                                                                                  100 µg 
                                                                                                                                                                                  Add to Cart
                                                                                                                                                                                  Reactivity: Influenza  
                                                                                                                                                                                  Application: ELISA  WB  
                                                                                                                                                                                    11048-RM07-50
                                                                                                                                                                                    11048-RM07-100
                                                                                                                                                                                    50 µg 
                                                                                                                                                                                    100 µg 
                                                                                                                                                                                    Add to Cart
                                                                                                                                                                                    Reactivity: Influenza  
                                                                                                                                                                                    Application: ELISA  WB  
                                                                                                                                                                                      11717-RP01-100
                                                                                                                                                                                      11717-RP01-200
                                                                                                                                                                                      100 µg 
                                                                                                                                                                                      200 µg 
                                                                                                                                                                                      Add to Cart
                                                                                                                                                                                      Reactivity: Influenza  
                                                                                                                                                                                      Application: ELISA  WB  IHC-P  IP  
                                                                                                                                                                                        11717-RP02-50
                                                                                                                                                                                        11717-RP02-100
                                                                                                                                                                                        50 µg 
                                                                                                                                                                                        100 µg 
                                                                                                                                                                                        Add to Cart
                                                                                                                                                                                        Reactivity: H6N1  
                                                                                                                                                                                        Application: ELISA  WB  IHC-P  IP  
                                                                                                                                                                                        • Slide 1
                                                                                                                                                                                        11723-T60-50
                                                                                                                                                                                        11723-T60-100
                                                                                                                                                                                        50 µg 
                                                                                                                                                                                        100 µg 
                                                                                                                                                                                        Add to Cart
                                                                                                                                                                                        Reactivity: H6N1  
                                                                                                                                                                                        Application: ELISA  
                                                                                                                                                                                          11723-RP01-200
                                                                                                                                                                                          11723-RP01-100
                                                                                                                                                                                          200 µg 
                                                                                                                                                                                          100 µg 
                                                                                                                                                                                          Add to Cart
                                                                                                                                                                                          Reactivity: Influenza  
                                                                                                                                                                                          Application: ELISA  WB  
                                                                                                                                                                                            11723-MM04-50
                                                                                                                                                                                            11723-MM04-100
                                                                                                                                                                                            50 µg 
                                                                                                                                                                                            100 µg 
                                                                                                                                                                                            Add to Cart