Anti-HA tag Antibody

Cat: 100028-MM15
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All HA tag Reagents
CRO Services
Product recommended

All HA tag Reagents
Anti-HA tag Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-HA tag Antibody
Validated applications
IP
Specificity
HA tag
Immunogen
A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Clone #15
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-HA tag Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
IP 4-8 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HA tag Antibody (Mouse Monoclonal antibody) Images
 HA Immunoprecipitation(IP) 18962

HA tag was immunoprecipitated using:

Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate

Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate

2 µL anti-HA tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HA tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

Product Description Host Clonality Application Catalog# (PDF)
Anti-HA tag Antibody Mouse Monoclonal WB,ELISA,FCM,ICC/IF,IF,IP 100028-MM10
Anti-HA tag Antibody Rabbit Polyclonal WB,IP 100028-T36
info info
添加购物车成功! 添加购物车失败!