Anti-HA tag Antibody

Cat: 100028-MM15
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All HA tag Reagents
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All HA tag Reagents
Anti-HA tag Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-HA tag Antibody
Validated applications
HA tag
A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA).
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Clone #15
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-HA tag Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
IP 4-8 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-HA tag Antibody (Mouse Monoclonal antibody) Images
 HA Immunoprecipitation(IP) 18962

HA tag was immunoprecipitated using:

Lane A:0.5 mg HA-ARG1-myc transfected 293 Whole Cell Lysate

Lane B:0.5 mg myc-ARG1-HA transfected 293 Whole Cell Lysate

2 µL anti-HA tag mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HA tag mouse monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 39 kDa

Observed band size: 39 kDa

Product Description Host Clonality Application Catalog# (PDF)
Anti-HA tag Antibody Mouse Monoclonal WB,ELISA,FCM,ICC/IF,IF,IP 100028-MM10
Anti-HA tag Antibody Rabbit Polyclonal WB,IP 100028-T36
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