Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb

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Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb (Rabbit Monoclonal antibody) General Information

Product name
Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb
Validated applications
ELISA,ELISA(Cap),FCM,HemagglutininInhibition(HI) (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Application notes
(Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.)
Specificity
H7N9 Hemagglutinin / HA
Has cross-reactivity in ELISA with
H7N9 (A/Hangzhou/1/2013) HA
H7N9 (A/Shanghai/2/2013) HA
H7N9 (A/Hangzhou/3/2013) HA
H7N9 (A/Shanghai/1/2013) HA
H7N9 (A/Anhui/1/2013) HA
H7N9 (A/Pigeon/Shanghai/S1069/2013) HA
H7N9 (A/Shanghai/4664T/2013) HA
H7N9 (A/Zhejiang/DTID-ZJU10/2013) HA
H7N9 (A/Zhejiang/1/2013) HA
H7N3 (A/turkey/Italy/214845/2002) HA
H7N7 (A/Netherlands/219/03) HA
H7N7 (A/chicken/Netherlands/1/03) HA
H7N7 (A/equine/Kentucky/1a/1975) HA
H7N8 (A/mallard/Netherlands/33/2006) HA
H7N1 (A/turkey/Italy/4602/99) HA
No cross-reactivity in ELISA with
H1N1 (A/California/07/2009) HA
H2N2 (A/Canada/720/2005) HA
H3N2 (A/Brisbane/10/2007) HA
H4N6 (A/mallard/Ohio/657/2002) HA
H5N1 (A/Anhui/1/2005) HA
H6N1 (A/northern shoveler/California/HKWF115/2007) HA
H8N4 (A/pintail duck/Alberta/114/1979) HA
H9N2 (A/Hong Kong/1073/99) HA
H10N3 (A/duck/Hong Kong/786/1979) HA
H11N2 (A/duck/Yangzhou/906/2002) HA
H12N5 (A/green-winged teal/ALB/199/1991) HA
H13N8 (A/black-headed gull/Netherlands/1/00) HA
H15N8 (A/duck/AUS/341/1983) HA
H16N3 (A/black-headed gull/Sweden/5/99) HA
Source
Monoclonal Rabbit IgG Clone #002
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb (Rabbit Monoclonal antibody) Validated Applications

Application Dilution
ELISA 0.1-0.2 μg/mL
FCM 0.5-2 μg/Test
ELISA(Cap) 0.5-4 µg/ml
Notes
ELISA(Cap): This antibody will detect Influenza A H7N9 HA / Hemagglutinin in ELISA pair set (Catalog: # SEK11082). In a sandwich ELISA, it can be used as capture antibody.
Hemagglutinin Inhibition (HI): Serially diluted antibody was pre-incubated with 4 units of hemagglutination titer of HA proteins for 1 hr at room temperature, the concentraion of antibody to stop 1% Guinea pig red cells hemagglutination is measured.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-H7N9 Hemagglutinin / HA Antibody, Hemagglutination Inhibition Activity, Rabbit MAb (Rabbit Monoclonal antibody) Images

Flow cytometric analysis of Purified anti-H7N9-HA antibody on 293 transfected cells. 293 cells were transfected with plasmid DNA of H7N7 (A/Netherlands/219/2003) Hemagglutinin (Cat.No. VG11082-G-N), then the cells were collected at 72 hours posttransfection, and the cell surface expression of HA were measured by flowcytometry.The transfected cells stained with Purified Rabbit anti-H7N9-HA ( Bold line hisgram, 1 μg /test), To demonstrate specificity of staining, the binding of 11082-R002 was blocked by the preincubation of the purified antibody with molar excess of recombinant Influenza A H7N9 (A/Shanghai/1/2013) Hemagglutinin (Left panel, 5 μg,Cat. No. 40104-V08H),and Influenza A H7N9 (A/Anhui/1/2013) Hemagglutinin (Right panel, 5 μg, Cat. No. 40103-V08H) for 1 hour (Dashed line hisgram), then stained with a FITC-conjugated second step antibody, grey line histogram represented negative control.

Hemagglutinin / HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
References
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37
  • A novel eight amino acid insertion contributes to the hemagglutinin cleavability and the virulence of a highly pathogenic avian influenza A (H7N3) virus in mice
    Author
    Sun, X;Belser, JA;Tumpey, TM;
    Year
    2016
    Journal
    Virology
    Application
    WB

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