Anti-DYKDDDDK (FLAG® epitope Tag) Antibody

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Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody
Validated applications
ICC/IF,IF
Specificity
DYKDDDDK (FLAG® epitope Tag)
Immunogen
A synthetic peptide corresponding to the flag-tag sequence (DYKDDDDK).
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #01
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution
ICC-IF 1:50-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal antibody) Images

Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38(Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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