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Anti-DYKDDDDK (FLAG® epitope Tag) Antibody, Mouse MAb

  • Anti-DYKDDDDK (FLAG® epitope Tag) Antibody, Mouse MAb, Immunohistochemistry
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  Antibody Product Information
Immunogen:A synthetic peptide corresponding to the flag-tag sequence (DYKDDDDK).
Clone ID:01
Ig Type:Mouse IgG2b
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
  Antibody IF Application Image
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38(Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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