Anti-Angiotensinogen Antibody

Cat: 10994-R001
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Anti-Angiotensinogen Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Angiotensinogen Antibody
Validated applications
WB,ELISA,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human Angiotensinogen
No cross-reactivity in ELISA with Human SerpinA1 / A1AT Human SerpinA3 / SERPINA3 / AACT Human SerpinA5 / Protein C inhibitor Human SerpinD1 / HC2 Human SerpinF2 / SERPINF2 Human SerpinG1 / C1IN Human SerpinI1 / Protease inhibitor 12 Human SerpinC1 /SERPINC1/ Antithrombin-III
Immunogen
Recombinant Human SerpinA8 protein (Catalog#10994-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human SerpinA8 / angiotensinogen (rh SerpinA8; Catalog#10994-H08H; NP_000020.1; Met 1-Ala 485).
Source
Monoclonal Rabbit IgG Clone #1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Angiotensinogen Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
ICC-IF 1:20-1:100  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Angiotensinogen Antibody (Rabbit Monoclonal antibody) Images
Human Angiotensinogen/SerpinA8 Immunohistochemistry(IHC) 3016
Immunofluorescence staining of Human AGT in Hela or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human AGT monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Mouse AGO2/Argonaute 2/EIF2C2 Immunoprecipitation(IP) 8247

SerpinA8 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SerpinA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SerpinA8 rabbit monoclonal antibody,at 1:200 dilution

Secondary antibody:

Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution

Developed using the DAB staining technique.

Performed under reducing conditions.

Predicted band size: 51 kDa

Observed band size: 51 kDa

Human Angiotensinogen/SerpinA8 Western blot (WB) 13317

Anti-SerpinA8 rabbit monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:53 kDa

Observed band size:53 kDa

Anti-Angiotensinogen Antibody Alternative Names
Anti-ANHU Antibody;Anti-SERPINA8 Antibody
Angiotensinogen Background Information

Angiotensinogen, also known as AGT and SerpinA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. Angiotensinogen can be schematically considered to consist of a combination of an angiotensin I (Ang I) function, located at the N-terminal end, and the presence of a serpin (serine protease inhibitor) structure at the opposite end. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD). Several serpins (antithrombin, maspin, pigment epithelial-derived factor, and kallistatin) have been recently shown to exert an antiangiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. Angiotensinogen/AGT and its renin-cleaved product, des(Ang I)AGT, are also angiogenesis inhibitors, both in vitro and in vivo at concentrations within the range of those observed in plasma. The Angiotensinogen products, that is angiotensin II and possibly angiotensin II-related products, have been found to act locally in modulating adipose tissue growth in an autocrine/paracrine manner. The transient or chronic overexpression of angiotensinogen in adipose tissue favors lipogenesis in adipocytes and leads to a 'vicious' circle whereby adipose tissue development is further increased.

Full Name
angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
References
  • Ailhaud G, et al. (2002) Angiotensinogen, adipocyte differentiation and fat mass enlargement. Curr Opin Clin Nutr Metab Care. 5(4): 385-9.
  • Corvol P, et al. (2003) Inhibition of angiogenesis: a new function for angiotensinogen and des(angiotensin I)angiotensinogen. Curr Hypertens Rep. 5(2): 149-54.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Angiotensinogen Antibody Rabbit Polyclonal ELISA 50337-RP01
    Anti-Angiotensinogen Antibody Rabbit Polyclonal WB,ELISA 50337-RP02
    Anti-Angiotensinogen Antibody Rabbit Polyclonal ELISA,IHC-P 10994-RP02
    Anti-Angiotensinogen Antibody Mouse Monoclonal ELISA(Cap) 10994-MM03
    Anti-Angiotensinogen Antibody Mouse Monoclonal FCM,ICC/IF,IF 10994-MM08
    Anti-Angiotensinogen Antibody (PE) Mouse Monoclonal FCM 10994-MM08-P
    Anti-Angiotensinogen Antibody (PE) Rabbit Monoclonal FCM 10994-R001-P
    Anti-Angiotensinogen Antibody Rabbit Monoclonal IHC-P 10994-R034
    Anti-Angiotensinogen Antibody Rabbit Polyclonal ELISA 10994-RP01
    Anti-Angiotensinogen Antibody (FITC) Rabbit Monoclonal FCM 10994-R001-F
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