SerpinA8 / Angiotensinogen / AGT Antibody

Serpin A8 / Angiotensinogen / AGT Antibody

Serpin A8 / Angiotensinogen / AGT Antibody Product Information

• Immunogen: Recombinant human SerpinA8 protein ( Catalog#10994-H08H )
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 6A10B4B5
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant human SerpinA8 / angiotensinogen ( rh SerpinA8 ; Catalog#10994-H08H ; NP_000020.1 ; Met 1 - Ala 485 ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Serpin A8 / Angiotensinogen / AGT Antibody Usage Guide

• Specificity: Human SerpinA8 / Angiotensinogen
• No cross-reactivity in WB and ELISA with

  Human SerpinA1 / A1AT

  Human SerpinA3 / SERPINA3 / AACT

  Human SerpinA5 / Protein C inhibitor

  Human SerpinD1 / HC2

  Human SerpinF2 / SERPINF2

  Human SerpinG1 / C1IN

  Human SerpinI1 / Protease inhibitor 12

  Human SerpinC1 / SERPINC1 / Antithrombin-III

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human SerpinA8 in WB. Using a DAB detection system, the detection limit for human SerpinA8 is approximately 1 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human SerpinA8. The detection limit for human SerpinA8 is 0.16 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Serpin A8 / Angiotensinogen / AGT Antibody Background

Angiotensinogen, also known as AGT and SERPINA8, is a member of the serpin family. It is an α-2-globulin that is produced constitutively and released into the circulation mainly by the liver. Angiotensinogen is a essential component of the renin-angiotensin system (RAS) and a potent regulator of blood pressure. It is expressed by the liver and secreted in plasma. Angiotensinogen is cleaved into three chains: Angiotensin-1 (Ang I), Angiotensin-2 (Ang II), and Angiotensin-3 (Ang III). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2. Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system. Defects in AGT are associated with susceptibility to essential hypertension and renal tubular dysgenesis (RTD).

References

1. Goodfriend, TL. et al.,1975, Circ. Res. 36: 38-48.
2. Weir, MR. et al., 1999, Am. J. Hypertens. 12: 205-13.
3. Rice, GI. et al., 2004, Biochem. J. 383: 45-51.
4. Jankowski, V. et al., 2007, Arterioscler Thromb Vasc Biol. 27(2): 297-302.